A high performance liquid chromatography method for the determination of mianserin in human serum was developed and validated. Doxepin was used as an internal standard. Mianserin was extracted from human serum using a liquid-liquid extraction with hexane:isoamyl alcohol (99:1, v/v). The sample was then dissolved in 0.05 M phosphoric acid (pH=3.0), and after separation on a Hichrom RPB (250 x 4....

PURPOSE To evaluate the physical characteristics and behavior of coils for endovascular therapy. METHODS Mechanically detachable coils were constructed with simple helical (4 mm X 10 cm and 8 mm X 30 cm) and pretzel shapes (4 mm X 5 cm) made from three metals using 0.003- and 0.004-in wire. Stiffness or pliability, frictional resistance, shape memory, and coil stability were evaluated in vitr...

Pairing rules have been developed to predict the sequence specificity of minor groove binding polyamides containing pyrrole (Py) and imidazole (Im) amino acids. An Im/Py pair distinguishes G.C from C.G and both of these from A.T/T.A base pairs. A Py/Py pair appears not to distinguish A.T from T.A base pairs. To test the extent of this degeneracy, the affinity and binding orientation of the hair...

A sensitive ESR method which allows a direct quantitative determination of nucleic acid binding affinities of proteins under physiologically relevant conditions has been applied to the gene 5 protein of bacteriophage fd. This was achieved with two spin-labeled nucleic acids, (ldT, dT)n and (lA,A)n, which served as macro-molecular spin probes in ESR competition experiments. With the two differen...

Radiolabeled Sendai virus was allowed to fuse with human erythrocytes and inside out vesicles were prepared from the erythrocyte membranes. Viral proteins incorporated into inside out vesicles were released from the membrane following treatment with various agents which perturb protein structure. Disruption products were analyzed by sucrose gradient centrifugation and sodium dodecyl sulfate-pol...

Angiotensin II (A II) and analogues were tested for their ability to restore electrical and mechanical activity to cardiac muscle preparations in which the fast Na+ channels had been inactivated by partial depolarization (22-27 mM K+) or by tetrodotoxin (TTX). The partially depolarized or TTX-blocked preparations were chosen because under these conditions electrical and mechanical responses are...

The GITC/CsCl method (1) is frequently utilized for the isolation of intact RNA. Here we describe the use of this method for the extraction of DNA binding proteins suitable for specific binding site blotting. Cells are tysed by guanidinium isothiocyanate and after the overnight ultracentrifugation step the supernatant of the gradient containing denatured cellular proteins is diarysed against bu...

To evaluate the pathogenic role of thromoboxane (Tx) in endotoxic shock, the potential protective effects of the Tx synthetase inhibitor, 7-(l-imidazolyl)-heptanoic acid (7-IHA) was assessed and compared to that of imidazole in the rat. 7-IHA (30 mg/kg) administered iv 30 minutes prior to Salmonella enteritidis endotoxin (20 mg/kg, iv) improved the survival rate from 42% (n = 24) at 5 hours in ...

developed a procedure, based on the affinity of all three enzymes for Cibacron Blue 3GA, which allows their simultaneous purification from a cell extract. E. coli K 12, EMG-2 was grown, harvested and disrupted as described by Miller & Stadtman (1972). After treatment with 1% (w/v) streptomycin sulphate, the cell extract was subjected to ammonium sulphate fractionation. The protein fraction prec...