3-Deoxy-D-manno-octulosonate 8-phosphate (KDOP) synthase and 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase catalyze similar phosphoenolpyruvate-utilizing reactions. The genome of Neisseria gonorrhoeae contains one gene encoding KDOP synthase and one gene encoding DAHP synthase. Of the two nonhomologous DAHP synthase families known, the N. gonorrhoeae protein belongs to the family ...

1. An improved method was developed for the assay of plant holo-(acyl carrier protein) synthase activity, using Escherichia coli acyl-(acyl carrier protein) synthetase as a coupling enzyme. 2. Holo-(acyl carrier protein) synthase was partially purified from spinach (Spinacia oleracea) leaves by a combination of (NH4)2SO4 fractionation and anion-exchange and gel-permeation chromatography. 3. The...

The analysis of the interaction of threose 4-phosphate and 2-deoxyerythrose 4-phosphate with 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DAH7PS) reveals previously unrecognised mechanistic differences between the DAH7PS-catalysed reaction and that catalysed by the closely related enzyme, 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS).

Abstract Menaquinone-7 (MK-7) is a kind of vitamin K2 playing an important role in the treatment and prevention cardiovascular disease, osteoporosis arterial calcification. The purpose this study to establish adaptive evolution strategy based on chemical modulator improve MK-7 biosynthesis Bacillus natto . inhibitor 5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase), glyphosate, was ch...

In Pseudomonas aeruginosa the initial enzyme of aromatic amino acid biosynthesis, 3-deoxy-D-arabinoheptulosonate 7-phosphate (DAHP) synthase, has been known to be subject to feedback inhibition by a metabolite in each of the three major pathway branchlets. Thus, an apparent balanced multieffector control is mediated by L-tyrosine, by L-tryptophan, and phenylpyruvate. We have now resolved DAHP s...

Acinetobacter sp. strain JC1 DSM 3803, a carboxydobacterium, grown on methanol was found to show dihydroxyacetone synthase, dihydroxyacetone kinase, and ribulose 1,5-bisphosphate carboxylase, but no hydroxypyruvate reductase and very low hexulose 6-phosphate synthase, activities. The dihydroxyacetone synthase was found to be expressed earlier than the ribulose 1,5-bisphosphate carboxylase. The ...

The conversions of 3-deoxy-D-arabino-heptulosonate ‘7phosphate (DAHP) to 3-enolpyruvylshikimate 5-phosphate (ES-S-P) and of N-(5’-phosphoribosyl)anthranilate (PRanthranilate) to indole3-glycerol phosphate (InGP) are catalyzed in uifro by two multienzyme complexes from Neurospora crassa. The rates of the over-all sequential reactions catalyzed by the two complexes were found to be greater than t...

The discovery of a drug requires over decade intensive research and financial investments – still has high risk failure. To reduce this burden, we developed the NICEdrug.ch resource, which incorporates 250,000 bioactive molecules, studied their enzymatic metabolic targets, fate, toxicity. includes unique fingerprint that identifies reactive similarities between drug–drug drug–metabolite pairs. ...

Carbamoyl-phosphate synthase I (pig liver) is modified at the cysteine residues 1327 and 1337 (numbered according to the rat sequence) in the presence of 5 mM-N-acetyl-L-glutamate with enhanced rate. ATP/Mg2+ (greater than or equal to 5 mM) protects against alkylation of these two cysteines and loss of activity. According to the results obtained by limited proteolysis of monobromobimane-modifie...