DNA primer sets, labeled with two fluorescent dyes to exploit fluorescence energy transfer (ET), can be efficiently excited with a single laser line and emit strong fluorescence at distinctive wavelengths. Such ET primers are superior to single fluorophore-labeled primers for DNA sequencing and other multiple color-based analyses [J. Ju, C. Ruan, C. W. Fuller, A. N. Glazer and R. A. Mathies (19...

Lasting immunity against Rickettsia tsutsugamushi, the causative agent of scrub typhus fever, has been demonstrated to be strain specific. Two protein antigens of 110 and 56 kilodaltons (kDa) have been shown to exhibit strain-specific epitopes. The 56-kDa scrub typhus antigen (Sta56) is an abundant outer membrane protein of R. tsutsugamushi and is an antigen often recognized by humans infected ...

MAP 4 is a ubiquitous microtubule-associated protein thought to play a role in the polymerization and stability of microtubules in interphase and mitotic cells. We have analyzed the behavior of protein domains of MAP 4 in vivo using chimeras constructed from these polypeptides and the green fluorescent protein (GFP). GFP-MAP 4 localizes to microtubules; this is confirmed by colocalization of GF...

We report the synthesis of ZnO nanotubes grown via the Ga-catalyzed vapor transport method at low temperature and their photocatalytic activity. The low melting point of Ga (29 °C) resulted in the growth of ZnO nanotubes at a low temperature of 80 °C, enabling us to use Kapton film or ITO glass as substrates. Structure analysis shows that the nanotube is single crystal and has a hollow structur...

A common physiological response of organisms to environmental conditions is variation in gene expression, especially true for genes encoding for heat shock proteins. In insects, this process has been examined for induced heat or cold stress. The putative long-term imprinted/acquired heat shock protein response due to unfriendly environmental conditions has been far less studied. The Drosop...

A substrate for a hypersensitive assay of ribonucleolytic activity was developed in a systematic manner. This substrate is based on the fluorescence quenching of fluorescein held in proximity to rhodamine by a single ribonucleotide embedded within a series of deoxynucleotides. When the substrate is cleaved, the fluorescence of fluorescein is manifested. The optimal substrate is a tetranucleotid...

BiOX (X = Cl, Br, I) photocatalysts with dominant (110) facets were synthesized via a mannitol-assisted solvothermal method. This is the first report on exposed facets-, size-, and defects-controlled synthesis of achieved by mannitol. polyol alcohol acted simultaneously as solvent, capping agent, and/or soft template. The mannitol concentration new morphology surface properties was investigated...

PURPOSE This clinical study using a specific technique was carried out to determine the clinical effectiveness of zygomatic implants under an immediate loading protocol. MATERIALS AND METHODS All patients treated between May 2000 and October 2006 who received zygomatic implants were included in this retrospective analysis. All patients were treated using the same surgical and restorative prot...

Multilocation yield trials on late duration (106-125 days) finger millet genotypes were conducted under early and late transplanting at Bhubaneswar and early direct-sown condition at Berhampur for three years 2004-2006, during kharif season. G×E interaction analysis of grain yield in AMMI (Additive Main and Multiplicative Interaction) model showed differential interaction of the genotypes in th...