This protocol describes the production of GST-Cbx7 fusion proteins from E. coli, originally developed in the recent publication (Zhen et al., 2016). The pGEX-6P-1-GST plasmids encoding the Cbx7 variants were transformed into BL21 competent cells. The fusion protein production was induced by isopropyl-beta-D-thiogalactopyranoside and they were purified by Glutathione Sepharose 4B. This protocol ...

Let G = Gn,n,p be the random bipartite graph on n+n vertices, where each e ∈ [n] appears as an edge independently with probability p. Suppose that each edge e is given an independent uniform exponential rate one cost. Let C(G) denote the expected length of the minimum cost perfect matching. We show that w.h.p. if d = np (log n) then E [C(G)] = (1 + o(1)) 2 6p . This generalises the well-known r...

A series of 5-carbomethoxybenzoxazole derivatives (6a-t) were prepared by using methyl-p-hydroxybenzoate. The identity of the compounds was confirmed on the basis of their elemental analysis and spectral data. In anti-MES test compounds 6b, 6d, 6h, 6j, 6m, 6p, 6q and 6s showed potent activity parallel to lipophilicity. Compounds 6b, 6d, 6k, 6n and 6s successfully passed the rotorod test without...

The Temperature Dependence of Spectral Walter Braun, Broadening in the Hg (6'So0 6P,) Multiplet Milton D. Scheer, and At High Optical Densities Victor Kaufman 313 Absolute Isotopic Abundance Ratio And Atomic Weight L. A. Machlan, J. W. Gramlich, Of a Reference Sample of Gallium L. J. Powell, and G. M. Lambert 323 Thermal Expansion of Platinum R. E. Edsinger, M. L. Reilly, and And Platinum-Rhodi...

Anticonvulsant potential and neurotoxicity of certain new imidazole-containing arylsemicarbazones 6a-p are reported. The test compounds 6a-p exhibited anticonvulsant activity mainly in the scPTZ screen. Compound 6p emerged as the most active surrogate displaying 100% protection at a dose level of 636 μ mol/kg in the scPTZ screen without any neurotoxicity. The assigned (E)-configuration of the t...

Pericentric inversion of chromosome 6 (6p+q-) was found in a girl with Rieger's syndrome and in her father. The only ocular signs in the father were prominent iris mounds and Schwalbe's line. The association of chromosomal anomalies with Rieger's syndrome indicates the need for a chromosome banding test in familial or sporadic patients with the syndrome and in patients with mild anomalies of th...

Brr2p is one of eight RNA helicases involved in pre-mRNA splicing. Detailed understanding of the functions of Brr2p and other spliceosomal helicases has been limited by lack of knowledge of their in vivo substrates. To address this, sites of direct Brr2p-RNA interaction were identified by in vivo UV cross-linking in budding yeast. Cross-links identified in the U4 and U6 small nuclear RNAs (snRN...