The carbohydrates of whole cells of group A, B, C, D, F, and G streptococci were analyzed with a highly sensitive gas chromatographic procedure. Characteristic chromatographic fingerprints were obtained for each group of streptococci grown in broth cultures or as single colonies on a blood agar plate. Rhamnose, glucose, and N-acetylglucosamine were major components of all the groups. Groups A, ...

Density functional theory calculations implemented by the SIESTA code are used to study the interactions of the saccharides N-acetylgalactosamine (GalNAc) and glucuronic acid (GlcA) with the (0001) and [Formula: see text] surfaces of the mineral hydroxyapatite (HAP). GalNAc and GlcA are the constituent monosaccharides of chondroitin, which is a glycosaminoglycan found in bone and cartilage, and...

Ricin toxin's binding subunit (RTB) is a galactose-/N-acetylgalactosamine (Gal/GalNac)-specific lectin that mediates uptake and intracellular trafficking of ricin within mammalian cells. Structurally, RTB consists of two globular domains, each divided into three homologous sub-domains (α, β, γ). In this report, we describe five new murine IgG monoclonal antibodies (mAbs) against RTB: MH3, 8A1, ...

Cell-to-cell interaction (coaggregation) between Propionibacterium acnes PK93 and Streptococcus sanguis DL1 was measured on saliva-coated hydroxyapatite beads (SHA) at bacterial concentrations between 1.3 X 10(6) and 6.7 X 10(8) cells per ml. Four hundredfold more DL1 than PK93 cells adhered to the saliva-coated beads, and the adherence of S. sanguis was proportional to cell input. SHA precoate...

The series: 9-methyl, 9-isopropyl, 9-cyclohexyl, 9,9-dimethyl, 9,9-diethyl, and the unsubstituted parent 2,7-bis (acetamido) fluorene were synthesized from the corresponding 9-substituted fluorenes. The two routes employed consisted of either (1) dinitration, reduction to the diamine, and diacetylation, or (2) Friedel-Crafts diacylation, then rearrangement via the Schmidt reaction. This provide...

Mucins are the principal components of mucus, and mucin glycosylation has important roles in defense, microbial adhesion, immunomodulation, inflammation, and cancer. Mucin expression and glycosylation are dynamic, responding to changes in local environment and disease. Potentially hundreds of heterogeneous glycans can substitute one mucin molecule, and it is difficult to identify biologically a...