Hoechst 33342, a bisbenzimidazole dye, binds to adenine/thymine rich regions in the minor groove of deoxyribonucleic acid (DNA). This dye induces apoptosis in BC3H-1 myocytes. The mechanism of Hoechst 33342-induced apoptosis was investigated. Inhibitors of ribonucleic acid (RNA) synthesis, protein synthesis, and serine or cysteine proteases failed to prevent BC3H-1 myocyte death induced by Hoec...

In this Letter, we investigated the dimerization behavior of the red-absorbing oxazine derivative MR121. Thereby a new method to determine absorption spectra of pure non-fluorescent H-type dye dimers is presented. Therefore, the amino acid lysine is doubly labeled with the fluorescent dye in order to obtain an absorption spectrum mainly caused by dimers. By subtracting the fraction arising from...

This study evaluated the ability of submerged aquatic plant Ceratophyllum demersum to remove Acid Blue 92 (AB92) dye. The effect some operational parameters such as reaction time, initial dye concentration, biomass, and pH, on removal efficiency was studied. Based Taguchi’s results, optimized conditions for were time 7 days, concentration 20 mg l-1, biomass 4 g, pH 5. Fourier-transform infrared...

Coomassie blue protein dye-binding assays are commonly used for quantitation of protein. They exploit the interaction between protein and Coomassie brilliant blue (CBB) G-250, the latter being solubilized in either ethanol/phosphoric acid (1) or perchloric acid (2). The Bradford assay (1) is the method of choice. Potential problems with these assays include non-linearity, a variable response to...

NiCr2O4 normal spinel nanostructures were prepared via hydrothermal treatment at 180 °C for 12 h in the presence of cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulphate (SDS) and poly vinylpyrrolidone-25000 (PVP-25000) as capping agents and subsequent calcination process at 500 °C for 3 h . In this method, [Ni(en)2(H2O)...

Mixed anaerobic bacterial consortia have been show to reduce azo dyes and batch decolourisation tests have also demonstrated that predominantly methanogenic cultures also perform azo bond cleavage. The anaerobic treatment of wool dyeing effluents, which contain acetic acid, could thus be improved with a better knowledge of methanogenic dye degradation. Therefore, the decolourisation of two azo ...

In the present study copper oxide nanoparticles (CuNPs) were synthesized via simple and eco-friendly green route using black tea extract . Characterization of (NPs) was undertaken. The characteristic absorption peak CuNPs in range (352-355) nm UV–Vis spectrum. Scanning electron microscopy (SEM) transmission (TEM) studies revealed morphological structural character NPs. particle size 25-50 nm. E...

One kind of adsorbent with a high adsorption capacity for anionic dyes was prepared using ionically and chemically cross-linked chitosan beads. A batch system was applied to study the adsorption behavior of one acid dye (MY, metanil yellow) and one reactive dye (RB15, reactive blue 15) in aqueous solutions by the cross-linked chitosan beads. The adsorption capacities was 3.56 mmol g(-1) (1334 m...

In this work, removal of C. I. Acid Red 14 was investigated by Pumice stone as a low cost adsorbent. Various parameters such as initial dye solution, contact time and pH were studied. Removal of dye were increased by increasing of contact time and initial dye solution and deceased by increasing of pH. Three isotherm models were studied. Linear and non-linear regression analyses were used fo...