The fusion of lysosomes and other granules with phagocytic vesicles (endo-fusion) and cell membrane (exocytosis) was simultaneously examined in non-phagocytosing guinea-pig polymorphonuclear leukocytes (PMNs). beta-Glucuronidase as a typical lysosomal enzyme, acid phosphatase as another lysosomal enzyme, and alkaline phosphatase as a specific granule enzyme were assayed. PMNs released these thr...

While studying the phosphoglyceric acid mutase of wheat germ, we noted marked phosphatase activity when extensively purified mutase preparations were tested with n-3-phosphoglyceric acid and D-Z, 3diphosphoglyceric acid as substrates (1). Since we had found low phosphatase activity for these reagents in many biological materials (2), there was the possibility, previously discussed (l), that the...

OBJECTIVES To assess the effect of topical application of melatonin to the gingiva on salivary fluid concentrations of acid phosphatase, alkaline phosphatase, osteopontin, and osteocalcin. STUDY DESIGN Cross-sectional study of 30 patients with diabetes and periodontal disease and 30 healthy subjects. Diabetic patients were treated with topical application of melatonin (1% orabase cream formul...

In this work, we have investigated the involvement of DmCatD, a cathepsin D-like peptidase, and acid phosphatase in the process of follicular atresia of Dipetalogaster maxima, a hematophagous insect vector of Chagas' disease. For the studies, fat bodies, ovaries and hemolymph were sampled from anautogenous females at representative days of the reproductive cycle: pre-vitellogenesis, vitellogene...

Histochemical methods have been devised Lo demonstrate certain metabolic activities of cells. By these techniques certain structures in a particular tissue are made to stand out in contrast to the other constituents. We have applied the methods for demonstrating alkaline and acid phosphatase activity to the study of the skin in leprosy. Pearse (5) has pointed out that these methods depend on in...

background: protective effect of emblica against radiation and cadmium induced biochemical changes in mouse kidney has been studied. materials and methods: adult male mice were divided into seven groups: i (shamirradiated), ii (cadmium chloride), iii (irradiated with 2 gy gamma rays), iv (radiation and cadmium chloride), v (cadmium chloride and emblica), vi (radiation and emblica), vii (radiati...

Background: Enzymes are the biological catalysts which accelerate the various cellular reactions. In general, the enzymes alkaline phosphatase (ALPase), acid phosphatase (ACPase), 5′-nucleotidase (5′-Nu) and glucose-6phosphatase (G-6-Pase) have important roles in metabolism. In the present study, a comparative account of the localization of activity of four enzymes acid phosphatase, alkaline ph...

In the course of synthesizing a number of aryl phosphate esters as substrates for prostatic acid phosphatase, polymeric phosphorylated products obtained by reaction of 4,4’-dihydroxybiphenyl with phosphorus oxychloride were found to be potent enzyme inhibitors. This report describes the preparation of these anionic polymers and some characteristics of their inhibition of prostatic acid phosphat...

Glycogen autophagy in newborn rat hepatocytes was studied by using enzyme determinations and electron microscopy. Cyclic AMP induced glycogen autophagy in these cells. Glycogen-hydrolyzing acid glucosidase activity increased whereas acid mannose 6-phosphatase activity decreased in the liver of these animals. Parenteral glucose, which prevents postnatal glucagon secretion and tissue cyclic AMP e...

Many phosphorus release experiments have been conducted in the water level fluctuation zone (WLFZ). However, effects of rhizospheres on risk soil remain unclear. In this study, twenty-four samples were collected from WLFZ Pengxi River, largest tributary northern Three Gorges Reservoir. Physicochemical properties, forms and phosphatase activity rhizosphere near-rhizosphere soils investigated. Bo...