1. The addition of small amounts of aqueous gentian violet, acriflavine, or acriviolet to suspensions of young cultures of B. anthracis reverses their Gram reaction, and diminishes their diameter about 40 per cent. 2. The time required for these changes varies with the strain of B. anthracis examined. 3. These changes are accompanied by a loss of weight. 4. Ninhydrin-positive substances are dem...

Resistant strains of Aspergillus nidulans were obtained by one-step selection on high concentrations of various inhibitors; each strain was mutant in a different gene conferring resistance to actidione (Act), p-fluorophenylalanine (p f ) , teoquil ( te) , iodoacetate (Iod) or malachite green (mg). Some mutant alleles have been firmly, others tentatively, located. For comparative purposes attemp...

The detection of Listeria monocytogenes from food is currently carried out using a double enrichment. For the ISO methodology, this double enrichment is performed using half-Fraser and Fraser broths, in which the overgrowth of L. innocua can occur in samples where both species are present. In this study, we analyzed the induction of phages and phage tails of Listeria spp. in these media and in ...

Colonial morphology mutants of Nocardia erythropolis were isolated following ultraviolet (UV) irradiation. The alleles rou-1/smo-1 were located by recombinant analysis and found to be linked to previously mapped characters. On the basis of recombinant class type patterns obtained from various selective characters it was postulated that the rou-1 allele may span a region of unique nucleotides in...

All of 41 naturally occurring coagulase-positive methicillin-resistant strains of Staphylococcus aureus isolated in various laboratories were resistant to several antibiotics and were lipase-negative. Most strains produced hemolysins, and 38 strains produced enterotoxin B. Acriflavine treatment of four strains resulted in elimination of resistance to methicillin and mercury; in one strain, resi...

The treatment of Streptomyces parvulus and Streptomyces antibioticus with acriflavine or novobiocin resulted in the loss of ability to produce actinomycin. The concomitant loss of ability to form aerial mycelium and the incidence of auxotrophic progeny (S. parvulus) were both low relative to the loss of the antibiotic-producing property. Protoplast fusion induced by polyethylene glycol 4000, us...

Sir,?In common justice to myself and to the manufacturers of Peracrina 303, I feel it my duty to comment shortly on the article by Dr. William Fletcher, published under the above title in the Indian Mcdical Gazette for November 1925. To begin with, Dr. Fletcher's disparagingly worded conclusion as to the composition of Peracrina 303, though apparently contradictory to the description given ?f t...