The chronic effects of ACTH and cortisol on mean arterial pressure (MAP) and related variables were studied in normotensive dogs and, subsequently, in the same dogs after they had been made hypertensive by chronic infusion of angiotensin II (All). MAP was recorded continuously, 24 hours/day, and sodium intake was 71 mEq/day. In both normotensive and hypertensive dogs, 8 to 10 days of ACTH infus...

Since no direct chemical method is at present available for the quantitation of ACTH secretion, indirect indices have, of necessity, been employed. Although many physiological and biochemical changes are associated with an increased activity of the pituitary-adrenal system, only a few of these are applicable as a method for the detection of such activity. Up to the present time, the detection o...

ACTH response in vivo and in vitro to synthetic ovine corticotropin-releasing factor (o-CRF) was examined in a bronchial carcinoid from a patient with ectopic ACTH syndrome. o-CRF, 1 microgram/kg iv bolus, scarcely increased plasma ACTH or cortisol on one occasion, but they showed a low response on retesting. On the other hand, 10(-8) and 10(-7) M of o-CRF significantly stimulated ACTH release ...

The appropriate dose and the ability of exogenous ACTH to mimic the physiological effects of a real stressor need to be determined. In Exp. 1, 25 pregnant Brahman heifers were injected i.v. with either 0 (saline), .125, .25, .5, or 1 i.u. of ACTH/kg BW. Plasma cortisol was determined in blood samples collected during a 5-h period, and an integrated cortisol response was calculated for each cow....

Objective: To determine neurodevelopmental and seizure prognoses in our patients with West syndrome receiving adrenocorticotropic hormone (ACTH) therapy, to identify the factors affecting these. Materials methods: We determined demographic factors, previous histories, ACTH use response times, etiological of 34 diagnosed clinic at 3-24 months therapy. also investigated their neurological develop...

cushing’s syndrome is a rare endocrine disease characterized by hypercortisolemia caused by excess acth secreted by the pituitary. distinguishing pituitary sources from the ectopic sources of acth is challenging because of the limited accuracy of noninvasive assays. the test that offers the highest sensitivity and specificity is bilateral inferior petrosal sinus sampling (bipss). in patients wi...

The late gestation plasma cortisol surge in the sheep fetus is critical for stimulating organ development and parturition. Increased adrenal responsiveness is one of the key reasons for the surge, however the underlying mechanisms are not fully understood. Our recent studies suggest that ACTH mediated increased expression of ACTH receptor (ACTH-R) and steroid acute regulatory protein (StAR) may...

Photolysis of rat adrenocortical cells in the presence of the photoreactive derivative [(2-nitro-5-azidophenylsulfenyl)Trp9]-adrenocorticotropic hormone (2,5-NAPS-ACTH) at 24 degrees C resulted in persistent activation of corticosterone production. The basal rate of steroidogenesis became maximal when photolysis was performed at 24 degrees C but remained the same as that of control cells when i...

Ectopic ACTH secretion in the setting of breast cancer is extremely rare but when present affects both the tumor's behavior and the incidence of complications. The patient, a 58-year-old woman, first presented with a mass in her left breast as well as multiple osseous metastases and a right femur fracture. Laboratory data revealed a hypokalemic alkalosis. Her plasma ACTH level was elevated. She...

Iodinated derivatives of adrenocorticotropic hormone (ACTH) were prepared with the use of NaI and chloramine-T and isolated by isoelectric focusing. The monoiodinated derivative (p1 7.6) was resolved into [monoiodoTyr2]ACTH and [monoiodoTyrZ3]ACTH by chromatography on columns of carboxymethylcellulose. Both monoiodo-AC’IH species exhibited only 30% of the maximum hormonal activity when assayed ...