Output of prostaglandin E2 (PGE2) from guinea pig ileal strips was detected by radioimmunoassay and the effect of adenosine and adenosine nucleotides on PGE2 output investigated in vitro. Adenosine triphosphate (ATP) and alpha, beta-methylene ATP, as well as adenosine, stimulate the output of PGE2 in a concentration dependent-manner. Potency of these compounds is in the order of alpha, beta-met...

The adenosine monophosphate (AMP)–activated protein kinase (AMPK) was initially identified as an enzyme acting “energy sensor” in maintaining energy homeostasis via serine/threonine phosphorylation when low cellular triphosphate (ATP) level sensed. AMPK participates catabolic and anabolic processes at the molecular levels is involved appetite-regulating circuit hypothalamus. signaling also modu...

Forrest, W. W. (University of Adelaide, Adelaide, South Australia). Adenosine triphosphate pool during the growth cycle in Streptococcus faecalis. J. Bacteriol. 90:1013-1018. 1965.-The adenosine triphosphate (ATP) pool has been studied throughout the growth cycle of Streptococcus faecalis. Normally, the pool is quantitatively related to the concentration of organisms and the growth rate, but de...

Abstract Funding Acknowledgements Type of funding sources: None. Background Adenosine (ADN) infusion may terminate focal atrial tachycardia (AT). However, the effect ADN on reentrant (RAT) is unclear and based case reports small series. These latter included mostly patients with cavotricuspid isthmus-dependent flutter provided conflicting results, RAT termination found when it involved septal o...

1. Stimulation of the vagal non-adrenergic inhibitory innervation caused the release of adenosine and inosine into vascular perfusates from the stomachs of guinea-pigs and toads.2. Stimulation of portions of Auerbach's plexus isolated from turkey gizzard caused the release of adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP).3. ATP, added to solutions p...

In adipocytes, adenylate cyclase is positively regulated by beta-adrenergic agents and negatively regulated by adenosine. Incubation of adipocytes with adenosine deaminase relieves the inhibition of adenylate cyclase by destroying the adenosine that the cells release into the medium. When adipocytes are incubated with adenosine deaminase and the beta-adrenergic agent isoproterenol, most of thei...

Exposure of human neutrophils to micromolar concentrations of both hydrolyzable and nonhydrolyzable purine nucleotides caused the generation of transient rises in intracellular calcium (Ca2+), Ca2+ fluxes across the membrane, and primed the cells for enhanced production of superoxide (O2-) when subsequently exposed to agonists such as FMLP and arachidonic acid. The neutrophils were most sensiti...

Whereas the chemotactic peptide, N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMet-Leu-Phe), induced NADPH-oxidase-catalyzed superoxide (O2-) formation in human neutrophils, purine and pyrimidine nucleotides per se did not stimulate NADPH oxidase but enhanced O2- formation induced by submaximally and maximally stimulatory concentrations of fMet-Leu-Phe up to fivefold. On the other hand, FMet-...

A procedure has been described for the purification of nucleoside diphosphatase from bovine liver microsomes. The purified enzyme is shown, by analysis in the ultracentrifuge and by polyacrylamide disc gel electrophoresis, to be nearly homogeneous. The enzyme has an s20,u, of 4.9 S. Thiamine pyrophosphatase was purified together with nucleoside diphosphatase. Evidence has been obtained which in...