Prothrombinase complex assembly, in real time, on platelets adherent to immobilized von Willebrand Factor (vWf) was examined by total internal reflection fluorescence spectroscopy (TIRFS). Electron microscopy showed that the platelets adhered to vWf in a largely unactivated state and could be activated by thrombin. Antibody binding to glycoprotein (GP) Ib and functional GPIIb-IIIa receptor mole...

Two morphological types of appendages, an anchor-like appendage and a peritrichate fibril-type appendage, have been observed on cells of an adhesive bacterium, Acinetobacter sp. strain Tol 5, by use of recently developed electron microscopic techniques. The anchor extends straight to the substratum without branching and tethers the cell body at its end at distances of several hundred nanometers...

Abstract Introduction Previous studies have shown that treatment of obstructive sleep apnea (OSA) with positive airway pressure (PAP) therapy in patients OSA and comorbid depression may improve response to antidepressant medication therapy. At the same time, scant evidence has examined impact PAP adherence depression. Patients adhere one be more likely other therapies or healthy behaviors a so-...

The Egyptian Book of the Dead, a collection of coffin texts, has long been thought by Egyptologists to describe the journey of the soul in the afterlife, or the Am Duat. In fact, the so – called Book of the Dead describes the invisible Substratum, the “black hole” form of matter to which all matter returns, and from which all matter arises. The hieroglyphics of the Papyrus of Ani, for example, ...

Using intuitive arguments, several investigators have proposed that the relative strengths of adhesion of cell to cell and of cell to substratum could determine whether or not monolayering and specifically contact inhibition of cell overlapping will occur. In the present communication, these 'strengths of adhesion' are given precise physical definitions, and the adhesive relationships which wou...

Malignantly transformed cells usually display a rosette-like morphology of substratum adhesions (called podosomes) and disorganized microfilaments, and are often associated with elevated production of chondroitin sulphate. We previously showed that many tissues and cells express alternatively spliced multiforms of the large chondroitin sulphate proteoglycan termed PG-M (versican is one of the s...

Cultures of human epidermal keratinocytes provide a useful experimental model with which to study the factors that regulate cell proliferation and terminal differentiation. One situation that is known to trigger premature terminal differentiation is suspension culture, when keratinocytes are deprived of substratum and intercellular contact. We have now investigated whether area of substratum co...