OBJECTIVE The need for an alternative to fetal bovine serum (FBS) is known to scientists and users involved in cell therapy or advanced therapy medicinal products. Human serum (huS) and platelet lysate (hPL) can be used as alternatives resulting in similar or even superior results concerning cell expansion. METHODS We developed protocols for the production of huS and two types of hPL and test...

the aim of this study was to investigate the interactions between rat intestine decellularized scaffold and human adipose derived mesenchymal stem cells. rat large intestine was dissected in fragments and decellularized by physicochemical methods. the scaffolds were loaded by human adipose derived mesenchymal stem cells expressing green fluorescent protein. microscopic sections were prepared fr...

Adipose-derived stromal cells (ASCs) are one of the most promising stem cell populations that differentiate into the mesodermal as well as neural lineages in vitro. In this study, we examined the neural differentiating potential of human ASCs by a neurosphere culture method. Neurospheres derived from human ASCs expressed Nestin and Musashi-1 genes, which are marker genes for neural stem cells. ...

UNLABELLED Adipose-derived stem cells (ASC) have received considerable attention in oncology because of the known direct link between obesity and cancer as well as the use of ASCs in reconstructive surgery after tumor ablation. Previous studies have documented how cancer cells commandeer ASCs to support their survival by altering extracellular matrix composition and stiffness, migration, and me...

BACKGROUND Adipose-derived stromal (stem) cells (ASC) have been shown to be of great therapeutic use in pre-clinical studies in diverse fields, but a standard expansion method has not been established. We investigated the effects of an endothelial growth medium (EGM-2) on ASC, focusing on proliferation and differentiation potentials. METHODS ASC were cultured in EGM-2 and DMEM. Doubling time ...

Introduction: UV light is the principal cause of photoaging, a kind aging. The problem arises because it less enjoyable. Utilizing secretome adipose stem cells (ASC), which rich in growth factors, could be novel anti-photoaging therapy. ASCs has been extensively examined for their role wound healing, but function photoaging just lately investigated. This study looked at changes epidermal and de...

This work describes the differentiation of adipose-derived mesenchymal stem cells (ASC) in a composite hydrogel for use as a vascularized dermal matrix. Our intent is that such a construct could be utilized following large-surface-area burn wounds that require extensive skin grafting and that are limited by the availability of uninjured sites. To develop engineered skin replacement constructs, ...

background: in recent years, adipose tissue, due to the stem cells contained within, has found a new special place in laboratory and clinical applications. these adipose‑derived stem cells (adscs) have the same characteristics of bone marrow mesenchymal stem cells (bmscs). although bone marrow (bm) is not easily accessible and its procurements may be painful, most patients possess excess fat wh...