OBJECTIVES Provide background for use of acquiring autologous adipose tissue as a tissue graft and source of adult progenitor cells for use in cosmetic plastic surgery. Discuss the background and mechanisms of action of closed syringe vacuum lipoaspiration, with emphasis on accessing adipose-derived mesenchymal/stromal cells and the stromal vascular fraction (SVF) for use in aesthetic, structur...

Abstract Background Novel therapeutic strategies for the healing of nonunion, which has serious effects on quality life patients, are needed. We evaluated effect local transplantation human stromal vascular fraction (SVF) cells fracture in a rat non-healing model and compared between freshly isolated (F) cryopreserved (C)-SVFs. Methods Non-healing was induced femur female immunodeficient rats (...

Macrophage infiltration is a critical determinant of high-fat diet induced adipose tissue inflammation and insulin resistance. The precise mechanisms underpinning the initiation of macrophage recruitment and activation are unclear. Macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine, displays chemokine-like properties. Circulating MIF levels are elevated during obesity how...

Adipose tissue allows for triacylglycerol storage in periods of energy excess and the subsequent use of triacylglycerol stores during energy deprivation with the lipogenic/lipolytic balance under tight hormonal control. Information is incomplete on the origins of adipose cells and adipose tissue. White adipose tissue is not detected during embryonic life or at birth in rats and mice, but is pre...

Leptin has been shown to reduce body fat in vivo. Adipocytes express the leptin receptor; therefore, it is realistic to expect a direct effect of leptin on adipocyte growth and metabolism. In vitro studies examining the effect of leptin on adipocyte metabolism require supraphysiological doses of the protein to see a decrease in lipogenesis or stimulation of lipolysis, implying an indirect actio...

Here we report that lean mice infected with the intracellular parasite Neospora caninum show a fast but sustained increase in the frequency of IFN-γ-producing cells noticeable in distinct adipose tissue depots. Moreover, IFN-γ-mediated immune memory could be evoked in vitro in parasite antigen-stimulated adipose tissue stromal vascular fraction cells collected from mice infected one year before...