The ingested nitrates sourced from tap water, food, chemicals and pharmaceuticals are converted to nitrites in the body surfaces by bacteria and then, the nitrite ions can lead the structural changing in hemoglobin. In the present work, aggregation of the purified hemoglobin in adult (HbA) and in fetus or newborn (HbF) in the presence of nitrite ions were studied. Hemoglobin aggregation was per...

Preoperative anemia and nadir hemoglobin (Hb) during cardiopulmonary bypass (CPB) have been identified as significant risk factors for blood transfusion during cardiac surgery. The aim of this study was to confirm the association between preoperative anemia, perioperative fluid management, and blood transfusion. In addition, the proportion of elective cardiac surgery patients presenting for sur...

Nuclear magnetic resonance studies of the contact-shifted spectra of heme protons in deoxyhemoglobin A from human adults show conclusively that oxygen binds to the alpha hemes in preference to the beta hemes. The preferential binding is produced in 10% hemoglobin solution at neutral pH by either a 15-fold molar excess of 2,3-diphosphoglycerate or a 5-fold molar excess of inositol hexaphosphate....

Human hemoglobin genes are located in α and β globin gene clusters in chromosomes 16 and 11, respectively. Different types of hemoglobin are synthesized according to the stage of development with fetal hemoglobin (α(2)γ(2)) (Hb F) being the main hemoglobin in the fetal period. After birth, there is a reduction (to about 1%) in Hb F levels and adult hemoglobin, Hb A (2α(2)β(2)), increases to mor...

Intracerebral hemorrhage (ICH) is a major cause of disability in adults worldwide. The pathophysiology of this syndrome is complex, involving both inflammatory and redox components triggered by the extravasation of blood into the cerebral parenchyma. Hemoglobin, heme, and iron released therein seem be important in the brain damage observed in ICH. However, there is a lack of information concern...

The N-terminal structure of adult Japanese monkey (macaca fuscata fuscata) hemoglobin and the N-terminal amino acid analyses of the tryptic peptides from this hemoglobin were performed. Hemoglobin, after removal of heme, was dinitrophenylated. The DNP-globin was hydrolyzed with HCl, and from the hydrolysate DNP-derivatives were extracted with ether or ethyl acetate. They were isolated and ident...