Antifungal activity of essential oils (EOs) of Zataria multiflora (thyme) and Syzygium aromaticum (clove) against Aspergillus flavus growth and aflatoxin B1 production was studied in potato dextrose agar (PDA) and potato dextrose broth (PDB), as well as in Iranian white cheese as a food model. The results showed that the inhibitory potential of EOs in the PDB medium is more than PDA medium. Clo...

effect of adding hibiscus sabdariffa (hs) in diet on performance and immune response of broilers was examined during aflatoxicosis using a total of 192 day-old ross 308 male chicks in a completely randomized design with four treatments, four replicates and 12 chicks per each replicate. treatments were include: 1. negative control (without additives), 2. positive control (2.5 mg aflatoxin b1/kg)...

A survey was conducted in the five rice-growing regions in Guyana to determine the presence of aflatoxins in multiple fractions of rice in June/October 2015 growing season. The fractions were paddy, steamed paddy, cargo rice, white rice and parboiled rice. Samples were analyzed by High Performance Liquid Chromatography. A subset of the samples was further analyzed by enzyme-linked immunosorbent...

Aflatoxins have long been suspected to be human hepatic carcinogens but no direct study was feasible until assays to measure individual aflatoxin exposure became available. We have used assays for urinary aflatoxin B1, its metabolites AFP1 and AFM1, and DNA-adducts (AFB1-N7-Gua) to assess the relation between aflatoxin exposure and liver cancer, as part of an ongoing prospective study of 18,244...

background: due to the high toxicity of aflatoxin and its effects on public health, determination of aflatoxin level in wheat flour samples in the golestan province, north of iran was investigated. to examine the effect of seasonal changes, summer and winter sampling was performed with standard sampling methods. methods: a total of 200 flour samples were collected from 25 factories. hplc method...

The multidrug resistance (mdr) genes encode P-glycoproteins, integral membrane proteins which function as drug efflux transporters. Exposure of animals in vivo and cells in vitro to a variety of xenobiotics leads to increased mdr1 gene expression and higher levels of P-glycoprotein. This response may protect cells from the cytotoxic effects of these compounds. In this investigation we functiona...

Citation: Aswani Kumar YVV, Renuka RM, Achuth J, Venkataramana M, Ushakiranmayi M and Sudhakar P (2018) Development of Hybrid IgG-Aptamer Sandwich Immunoassay Platform for Aflatoxin B1 Detection and Its Evaluation Onto Various Field Samples. Front. Pharmacol. 9:271. doi: 10.3389/fphar.2018.00271 Development of Hybrid IgG-Aptamer Sandwich Immunoassay Platform for Aflatoxin B1 Detection and Its E...

Background & Aims: Aflatoxins are a group of toxic compounds found in most plant products such as pistachio, corn, spices, wheat, and rice. This study was carried out to detect the presence of aflatoxin B1 (AFB1) in samples of spices collected from Qaemshahr in Mazandaran province, Iran. Methods: This was a cross-sectional study on the presence of AFB1 in 18 samples of spices including chilli (...

A high-affinity IgM monoclonal antibody specific for aflatoxins was covalently bound to Sepharose 4B and used as a preparative column to isolate aflatoxin derivatives from the urine of people and experimental animals who had been exposed to the carcinogen environmentally or under laboratory conditions. Aflatoxin levels were quantified by radioimmunoassay and high-performance liquid chromatograp...