objectives: osteoblasts are the most important cells in the osseointegration process. despite years of study on dental implants, limited studies have discussed the effect of saliva on the adhesion process of osteoblasts to implant surfaces. the aim of this in vitro study was to evaluate the effect of saliva on morphology and differentiation of osteoblasts attached to implant surfaces. materials...

We describe two new methods for the separation and quantification of the bone and liver isoenzymes of alkaline phosphatase (EC 3.1.3.1) in plasma. In the first, we use wheat-germ lectin to precipitate the bone isoenzyme. About 80% of this, but minimal liver isoenzyme, is precipitated. The activity of the bone isoenzyme is calculated from measuring the alkaline phosphatase activity in the precip...

High pH values have been found necessary for optimum activity of alkaline phosphatase in vitro. The pH values in the living cell structures, on the other hand, are near neutrality: cytoplasm 6.9, nucleus 7.6 (l-3). This definite difference between the pH required for optimum activity and the pH of the living cell indicates that alkaline phosphatase is not optimally active in viva. However, the ...

The administration of 1,25-dihydroxyvitamin D, (1,25(OH),D,) to rachitic chicks produces an increase in (a) RNA and protein synthesis, (b) calcium binding protein (CaBP) concentration, and (c) alkaline phosphatase activity in the duodenum. These events occur concomitantly with enhanced calcium transport. We inhibited RNA and protein synthesis in rachitic chicks and measured the subsequent respo...

CYP3A4 probably has the broadest catalytic activity of any cytochrome P450. It is a crucial task to test new drug candidates in a reliable system for their ability to induce expression of this enzyme. Firstly, a total of 300 bp core distal enhancer of CYP3A4 XREM region (-7972/-7673) were amplified from human genomic DNA. The PCR product was then ligated into a human secretory alkaline phosphat...

In the present study phosphatase levels ( Acid and Alkaline ) were compared with sperm count and motility in 100 subjects with varying conditions. All the subjects presented were between 21-45 years of age. Samples of semen were collected from 100 cases comprising of the different grades of sperm concentration and motility. Human seminal fluid contains significant amount of acid phosphatase and...

A total o f 28 strains o f several species o f Rhizobium were screened for the presence and regulation of the enzyme alkaline phosphatase. In contrast to all other species o f Rhizobium, the activity of this enzyme was absent in R. trifolii. D ifferent levels o f alkaline phosphatase were observed in various species o f Rhizobium. Both, species as well as strain specific differences were found ...

Enzymes associated with release of iron from internalized ferrated siderophore (ferrisiderophore reductase), with damage to the cell at high iron concentration (superoxide dismutase) and siderophore synthesis (alkaline phosphatase), were examined in 3 test fungi viz., Aspergillus sp. ABp4, Aureobasidium pullulans and Rhizopus sp. Extracellular ferrisiderophore reductase activity was present in ...

on the response of bone alkaline phosphatase to parathyrin. The results of a typical experiment showing percentage change in alkaline phosphatase activity compared with the hormone-free control are presented in Fig. 2(b). Tissue from 1-day-old animals was unresponsive to parathyrin, that from the 3-day-old animal showed decreased alkaline phosphatase activity and those from 5and 6-day-old anima...

The administration of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) to rachitic chicks produces an increase in (a) RNA and protein synthesis, (b) calcium binding protein (CaBP) concentration, and (c) alkaline phosphatase activity in the duodenum. These events occur concomitantly with enhanced calcium transport. We inhibited RNA and protein synthesis in richitic chicks and measured the subsequent respo...