An expressed sequence tag (EST) library was established from the hypopharyngeal glands of Apis cerana. Sixty-six recombinant clones, possessing inserts > 500 bp, were randomly selected and unidirectional sequenced. Forty-two of these (63.6%) were identified as homologues of Major Royal Jelly Proteins families 1, 2, 3, and 4 of A. mellifera (AmMRJP) for which MRJP1 was the most abundant family. ...

To isolate melanoma Ags recognized by T cells, cDNA libraries made from melanoma cell lines were screened with four CTLs derived from tumor infiltrating lymphocytes (TIL) that were able to recognize melanoma cells in a HLA-A1, -A2, or -A3 restricted manner. Although cDNAs encoding the previously identified melanoma Ags, tyrosinase and gp100, were isolated, these TIL were found to recognize prev...

Fluid-dynamic conditions that are compatible with tensile stress on the bonds between platelet glycoprotein Ibalpha and immobilized Von Willebrand factor A1 domain (VWF-A1) led to Ca++ release from intracellular stores (type alpha/beta peaks), which preceded stationary platelet adhesion. Raised levels of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate inhibited these [C...

Tektins are a class of proteins that form filamentous polymers in the walls of ciliary and flagellar microtubules, and they may also be present in centrioles, centrosomes and mitotic spindles. We report here the cloning and sequencing of a cDNA for ciliary tektin B1. Comparison of the predicted amino acid sequence of tektin B1 with the previously published sequence for tektin A1 reveals several...

Hromatska aberacija je optički defekt koji uzrokuje da se svetlosni zraci različitih talasnih dužina fokusiraju na različite tačke duž optičke ose sočiva. Manifestuje kao traka jedne boje prelazima okvira oko kontrastnih ivica fotografiji. Postoje dve vrste hromatskih aberacija: longitudinalne i transverzalne. Transverzalna hromatska koja javlja zato što uvećanje slike razlikuje u zavisnosti od...

5,981,180 A 11/1999 Chandleret a1. 6,046,807 A 4/2000 Chandler 6,139,800 A 10/2000 Chandler 6,268,222 B1 7/2001 Chandleret a1. 6,366,354 B1 4/2002 Chandler 6,449,562 B1 9/2002 Chandler et a1. 6,514,295 B1 2/2003 Chandleret a1. 6,524,793 B1 2/2003 Chandleret a1. 6,592,822 B1 7/2003 Chandler 6,599,331 B2 7/2003 Chandleret a1. 6,632,526 B1 10/2003 Chandler 6,649,414 B1 11/2003 Chandler et a1. 6,69...

Tumor necrosis factor-alpha (TNF-alpha) is elevated in the failing heart. Very little is known about regulation of TNF-alpha in cardiomyocytes. TNF-alpha expression by macrophages is diminished by adenosine. Therefore, we hypothesized that a similar mechanism might occur in the heart. Neonatal rat myocytes were stimulated with lipopolysaccharide (LPS), and TNF-alpha was measured by ELISA. In th...

introduction: stroke is one of the most important reasons of death. hence, trials to prevent or lessen the complications originated by stroke are a goal of public health worldwide. the ischemia-reperfusion causes hypoxia, hypoglycemia and incomplete repel of metabolic waste products and leads to accumulation of free radicals triggering neuronal death. the a1 adenosine receptoras an endogenous l...