Young bean plants (Phaseolus vulgaris L. var Saxa) were fed with three different types of inorganic nitrogen, after being grown on nitrogen-free nutrient solution for 8 days. The pattern of (14)CO(2) fixation was investigated in photosynthesizing primary leaf discs of 11-day-old plants (3 days with nitrogen source) and in a pulse-chase experiment in 13-day-old plants (5 days with nitrogen sourc...

Ammonium assimilation in Escherichia coli is regulated by two paralogous proteins (GlnB and GlnK), which orchestrate interactions with regulators of gene expression, transport proteins, and metabolic pathways. Yet how they conjointly modulate the activity of glutamine synthetase, the key enzyme for nitrogen assimilation, is poorly understood. We combine experiments and theory to study the dynam...

New evidences have recently emerged from studies in yeast and in higher eukaryotes showing the importance of nutrient balance in dietary regimes and its effects on longevity regulation.We have previously shown that manipulation of ammonium concentration in the culture and/or aging medium can drastically affect chronological lifespan (CLS)of Saccharomyces cerevisiae, especially in amino acid res...

In response to nitrogen starvation, diploid cells of the budding yeast Saccharomyces cerevisiae differentiate into a filamentous, pseudohyphal growth form. This dimorphic transition is regulated by the Galpha protein GPA2, by RAS2, and by elements of the pheromone-responsive MAP kinase cascade, yet the mechanisms by which nitrogen starvation is sensed remain unclear. We have found that MEP2, a ...

This study contributes to the improvement of low-cost biotechnology for wastewater treatment in constructed wetlands (CWs). Constructed wetlands are energy efficient engineered systems that mimic the treatment processes of natural wetlands, removing polluting organic matter, nutrients, and pathogens from water. The aim of this study was to investigate the advisability of the inoculation of hori...

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The fission yeast Schizosaccharomyces pombe undergoes a switch from yeast to filamentous invasive growth in response to certain environmental stimuli. Among them is ammonium limitation. Amt1, one of the three ammonium transporters in this yeast, is required for the ammonium limitation-induced morphological transition; however, the underlying molecular mechanism remains to be understood. Cells l...

The effect of nitrogen status on phosphorous uptake and translocation was examined in 6-day-old dark-grown decapitated maize seedlings exposed to 25 micromolar phosphorous. Transfer to complete solutions containing 1 millimolar ammonium resulted in an increase in phosphorous uptake rate after 6 to 8 hours. The stimulus remained effective for at least 5.5 hours upon subsequent transfer to nitrog...

The effective management of high ammonium containing wastewater is important for the sustainable development of the wastewater industry. A pre-denitrification and post-nitrification two-sludge system was proposed to treat high ammonium containing wastewater with low carbon-to-nitrogen (C/N) ratios. In the system, pre-denitrification was adopted to use organic carbon in raw wastewater efficientl...

Background & Aim:Peppermint known for its healing properties is a plant from the Labiatae family. Currently, different rates of nitrogenous fertilizers are used in production of peppermint while there is no precise information available about how much of nitrogen fertilizer is the optimum rate for this plant. The main purpose of this research was to evaluate the response of pep...