Endocannabinoids have been implicated in the mechanisms of implantation, maintenance of pregnancy, and parturition in women. Intrauterine prostaglandin production and actions are also critical in each of these mechanisms. Hence, we have evaluated the effects of cannabinoids on prostaglandin biosynthesis by human gestational membranes. Explants of term amnion and choriodecidua were established a...

UNLABELLED Meshed, split-thickness skin grafts, especially when required to be widely spread, do not obtain immediate biologic closure. In patients with burns that cover a large percentage of the body surface area, this leaves the patient at risk for metabolic problems and life-threatening infection. OBJECTIVE The purpose of this study was to determine whether amnion-derived cellular cytokine...

BACKGROUND Wound coverage for second-degree burns remains a clinical challenge. Human amniotic membranes have been used for many years in the treatment of burns; however, no large prospective clinical trials have been published. In this article, we present a novel and standardized procurement and processing method for amnion and investigate, whether the use of this biological dressing is safe a...

OBJECTIVE Wound repair is a complex integration of dynamic processes mediated by humeral messages controlling the levels of cytokines, growth factors, and matrix metalloproteinases in the wound space. Isolated growth factors and growth factor combinations have been used to accelerate wound healing with limited success. A cellular cytokine solution can be collected by harvesting the proteins rel...

The growth characteristics, nutritional requirements, and metabolic activities of primary explants (chiefly amnion) and transformed human cells (derived from amnion, conjunctiva, and cervical cancer) were compared. Major differences observed are as follows: 1. Five types of human tissue when freshly explanted in vitro were found to have varying but limited growth potential in contrast to the au...

Higher cyclorrhaphan flies including Drosophila develop a single extraembryonic epithelium (amnioserosa), which closes the germband dorsally. In most other insects two extraembryonic epithelia, serosa and amnion, line the inner eggshell and the ventral germband, respectively. How the two extraembryonic epithelia evolved into one is unclear. Recent studies have shown that, in the flour beetle Tr...

The development of polypoidal structures, derived from organization of amniotic squamous debris, in the middle-ear cavity of 3 infants is described. The lesions mimicked the development of amnion nodosum lesions of the placenta and were attached to either the tympanic membrane or the head of the stapes. They were accompained by atypical metaplastic changes in the middle-ear epithelium. While th...

In an earlier report (5), it was shown that under specified conditions the application of high hydro-static pressure to FL amnion ceils resulted in an increase of measurable endogenous adenine nucleotides , although the more slowly metabolizing primary amnion cells showed no such increase 408 BRIEF NOTES

Source (months) Serially propagated, long-term: 11ni iMii embryonal intestinal epithelium (Henle) (29) 35 Human liver (Chang) (6) 43 Human conjunctiva (Chang) (6) 43 Human carcinoma of nasopharynx (KB) (Eagle) (15) 36 Serially propagated, recently isolated: Human thymus (Foley) 17.5 Human amnion (Foley) 15.5 First in vitro passage, not serially propagated: Rhesus monkey kidney (Kagle, Foley) 0....