نتایج جستجو برای: amperometry

تعداد نتایج: 456  

Journal: :Neuron 2007
Ke-Ming Zhou Yong-Ming Dong Qian Ge Dan Zhu Wei Zhou Xian-Guang Lin Tao Liang Zheng-Xing Wu Tao Xu

The nematode C. elegans provides a powerful model system for exploring the molecular basis of synaptogenesis and neurotransmission. However, the lack of direct functional assays of release processes has largely prevented an in depth understanding of the mechanism of vesicular exocytosis and endocytosis in C. elegans. We address this technical limitation by developing direct electrophysiological...

Journal: :Biosensors & bioelectronics 2007
Yu-Chen Tsai Jing-Dae Huang Chian-Cheng Chiu

A novel amperometric ethanol biosensor was constructed using alcohol dehydrogenase (ADH) physically immobilized within poly(vinyl alcohol)-multiwalled carbon nanotube (PVA-MWCNT) composite obtained by a freezing-thawing process. It comprises a MWCNT conduit, a PVA binder, and an ADH function. The measurement of ethanol is based on the signal produced by beta-nicotinamide adenine dinucleotide (N...

Journal: :Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 2010
Hajime Katano Kohei Uematsu Hirosuke Tatsumi Toshihide Tsukatani

The reaction of free chlorine with tertiary ammonium or amine compounds in aqueous solution was studied by the amperometry at a rotating Pt-disk electrode. The amperometric method can be applied to follow the concentration of free chlorine (c(Cl)) even in the presence of chloramine species. By addition of mono- and dibutylammonium to the solution containing free chlorine, the step-like decrease...

Journal: :Langmuir : the ACS journal of surfaces and colloids 2015
Charles G Cranfield Taren Bettler Bruce Cornell

The nanoscale spacing between a tethered lipid bilayer membrane (tBLM) and its supporting gold electrode can be utilized to determine the polarity selectivity of the conduction of ion channels and ion carriers embedded in a membrane. The technique relies upon a bias voltage sequestering or eliminating ions, of a particular polarity, into or out of the aqueous electrolyte region between the gold...

Journal: :Neuron 2001
Abdeladim Elhamdani H.Clive Palfrey Cristina R. Artalejo

To what extent the quantal hypothesis of transmitter release applies to dense-core vesicle (DCV) secretion is unknown. We determined the characteristics of individual secretory events in calf chromaffin cells using catecholamine amperometry combined with different patterns of stimulation. Raising the frequency of action potential trains from 0.25-10 Hz in 2 mM [Ca(2+)]o or [Ca(2+)]o from 0.25-7...

Journal: :Journal of neuroscience methods 2009
Hao Zhang Shih-Chieh Lin Miguel A L Nicolelis

Simultaneous acquisition of in vivo electrophysiological and neurochemical information is essential for understanding how endogenous neurochemicals modulate the dynamics of brain activity. However, up to now such a task has rarely been accomplished due to the major technical challenge of operating two independent recording systems simultaneously in real-time. Here we propose a simpler solution ...

2017
Hongying Liu Lingyan Weng Chi Yang

The incorporation of nanomaterials into electrochemical sensors is an attractive approach towards the improvement of the sensitivity of amperometry and also can provide improved sensor selectivity and stability. This review (with 137 references) details the current state of the art and new trends in nanomaterial-based electrochemical sensing of hydrogen peroxide (H2O2), hydrogen sulfide (H2S) a...

2010
Francesco Crespi

Amperometric studies have indicated that substance P as well as NMDA stimulates release of NO in rat aortic rings. These data have been confirmed by functional observations of vaso-relaxant action of NMDA within noradrenaline pre-contracted aortic rings, supporting the presence of NMDA receptor in rat aortic rings. It is known that the enzyme endothelial NO synthase (eNOS) mediates vasodilatati...

Journal: :Neuron 2015
Joshua E. Motelow Wei Li Qiong Zhan Asht M. Mishra Robert N.S. Sachdev Geoffrey Liu Abhijeet Gummadavelli Zaina Zayyad Hyun Seung Lee Victoria Chu John P. Andrews Dario J. Englot Peter Herman Basavaraju G. Sanganahalli Fahmeed Hyder Hal Blumenfeld

Impaired consciousness in temporal lobe seizures has a major negative impact on quality of life. The prevailing view holds that this disorder impairs consciousness by seizure spread to the bilateral temporal lobes. We propose instead that seizures invade subcortical regions and depress arousal, causing impairment through decreases rather than through increases in activity. Using functional magn...

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 2000
T L Colliver S J Pyott M Achalabun A G Ewing

It has been well established that the volume of secretory vesicles can be modulated. However, we present the first data demonstrating that the amount of transmitter in a vesicle can regulate its volume. Amperometry and transmission electron microscopy have been used to determine that l-3,4-dihydroxyphenylalanine and reserpine increase and decrease, respectively, the volume of single pheochromoc...

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