this study involves in vitro androgenesis of zea maysl. via anther culture. combination of two embryoinduction media (imss & ypm) in presence of different colchicine concentrations (0, 100, 200, 250, 300and 400 mg/l ) in the pretreatment medium (iml) andpretreatment duration (0, 3, 6 and 9 days) in twogenotypes (dh5×dh7 and eth-m82) were tested.after colchicine pretreatment, anthers were tr...

The objective of this study was to estimate genetic parameters and to investigate the type of gene action in controlling androgenesis in wheat. Two wheat cultivars of Grebe and Houtman were reciprocally crossed with two synthetic genotypes of Do1 and Pol and then a complete set of the parents, F1, reciprocal F1 (RF1), F2 and back-cross generations (BC1 and BC2) of each cross were used for anthe...

This study involves in vitro androgenesis of Zea mays L. via anther culture. In the first experiment, three embryo induction media were tested in ETH-M82 genotype of maize. The results showed that the embryo structures were established 2 to 3 weeks after culturing the anthers. The effect of induction medium was signifi­cant for embryo frequencies. The obtained results confirm that the induction...

androgenesis via anther culture or microspore culture is one of the current methods for producing haploid and double haploid plants in maize. to use of this method in maize breeding program it should be able to regenerate enough plantlets. anthers culture usually is carried out indirectly via callus induction and regeneration on at least two different media. in this study a responsive genotype,...

haploid microspore-derived embryos (mdes) of wheat were obtained by in vitro androgenesis. these  embryos were employed to evaluate the transient expression of gus gene (uida) following particle bombardment. using the bio-rad pds-1000/he system, the physical parameters including rupture disk pressure (900, 1100 and 1350 psi); microprojectile travel distance (6 and 9 cm); gold particles size (0....

This study aimed to establish a compelling approach for inducing embryogenesis through in vitro anther culture Scarlet eggplant (Solanum aethiopicum L.), the most consumed and popular among indigenous vegetables on African continent. While androgenesis has been favorably employed brinjal melongena L.) breeding, there no attempt induce large germplasm of its relative, Solanum aethiopicum. In two...

Doubled haploid (DH) technology remarkably accelerates the crop breeding by obtaining homozygous lines in a single generation. The present study was targeted generating plants through androgenesis. Anthers from immature flower buds of six tomato genotypes viz., LE-1230, LE-1236, LE-1256 TLCV 2, PKM 1 and TNAU hybrid CO 3 were used for induction haploids. A preliminary based on callus frequency ...

several methods are available to produce haploid plants. one of the most efficient methods is especially microspore culture. stress as a triggering factor is essential for embryogenesis induction in microspores. in the first experiment, the effect of different thermal stresses including cold treatments and heat stress and the control were evaluated. in the second experiment, the effect of 2,4-d...