Introduction: Sambiloto (Andrographis paniculata) is an antidiabetic medicinal plant that acts by inhibiting the ?-amylase enzyme. Andrographolide, active compound of sambiloto leaf, insoluble in water but dissolves ethanol. Aim: This study compared vitro activity aqueous extract and ethanolic leaf with Methods: The inhibitory test enzyme was carried out using ultraviolet-visible spectrophotome...