background: visceral leishmaniasis is systematic serous parasitic disease with pub­lic health importance. zoonotic form of visceral leishmaniasis is wide spread in mediterranean basin and south america regions. direct agglutination test (dat) is an accurate, reliable and non-expensive serological test for the diagnosis of visceral leishmaniasis in human and canines but the antigen preparation i...

background: echinococcosis or hydatid disease is a zoonotic infection caused by larval (metacestode) stages of cestodes belonging to the genus echinococcus, family taeniidae . we aimed to subclone antigen b gene in pqe-30 plasmid, its expression, and purification. methods: we subcloned hi gene into pqe-30 expression vector. the recombinant vector was transformed into e. coli , m15 and mass cult...

background and objective: gliomas are the most common primary brain tumors. despite therapeutic advances, the majority of gliomas do not respond to either chemo or radiotherapy. cd117, the gene product of c-kit has been expressed in cells of glial tumors. because gastrointestinal stromal tumors (gists) that express cd117 respond dramatically to treatment with tyrosine kinase inhibitors, identif...

immune genes of the major histocompatibility complex (mhc) are among the most polymorphic genes in the vertebrate genome. due to their polymorphic nature, they are often used to assess the adaptive genetic variability of natural populations. this study describes the first molecular characterization of 13 partial mhc class iib sequences from three european ranid frogs.  the utility of previously...

regulation of normal cell growth and turnover is balanced between cell pro¬liferation, cell differentiation and apoptosis. a disruption of this balance is thought to be an important event leading to carcinogenesis .one of the effector molecules in apoptosis is fas antigen . cross-linking of fas by its ligand (fas l) or agonistic anti fas antibodies induces apoptosis of cells expressing fas on t...

in the last decades researchers had focused on developing a vaccine against tick based on protective antigen. recombinant vaccines based on concealed antigen from boophilus microplus have been developed in australia and cuba by the name of tickgard and gavac (de la fuente and kocan, 2006). further studies on this antigen have shown some extent of protection against other species (de vos et al.,...

Abstract Self antigen-specific T cells are prevalent in the mature adaptive immune repertoire. At steady state, these regulated by various mechanisms of peripheral tolerance. Characterizing activation self during inflammation may shed crucial insights into pathogenesis autoimmunity. Utilizing an experimental animal model where mice express three cell epitopes (2W, gp66, and OVA) a tissue restri...

cystic hydatid disease (hydatidosis) is the most serious tape-worm infection prevalent in the cattle and sheep raising area of the world. hydatidosis in man (as an accidental host) caused by infection with the ova containing larval stage of echinococcus spp. in the last decade different techniques have been employed for the serological diagnosis of hydatid disease, such as iha, ifa, elisa, ccle...

background: the study aimed to examine the association between human leukocyte antigen (hla) class ii allele frequencies and breast cancer in iranian women. methods: a total of 100 patients with pathologically proven breast cancer who referred to cancer institute were randomly selected and compared with a group of age matched healthy blood donors (n = 80). hla class ii alleles were determined b...

background: antigen b (agb) is frequently used for immuno-diagnosis of hu-man cystic echinococcosis (ce). echinococcus granulosus agbs show a high degree of genetic variability in different hosts or in different ce endemic areas. the present study aimed to evaluate the genetic polymorphisms of encoding antigen b2 gene (agb2) among different iranian isolates of e. granulosus. methods: a total of...