a murine igm monoclonal antibody (ma-2c6) with κ-light chains directed against an antigenic determinant of outer surface protein a (ospa) of the lyme disease spirochete, borreliaburgdorferi, is produced. this antibody could bind specifically to ospa antigen of several isolates of b. burgdorferi, but not to the non-lyme disease bacteria such as t. pallidum and b. hermsii. antibody ma-2c6 was pur...

King, Sylvia (Hektoen Institute, Chicago, Ill.) and Esther Meyer. Gel diffusion technique in antigen-antibody reactions of Actinomyces species and "anaerobic diphtheroids." J. Bacteriol. 85:186-190. 1963.-The Ouchterlony agar gel diffusion test was used to establish antigenic patterns produced by Actinomyces israelii, A. bovis, and A. naeslundii, as compared with those of "anaerobic diphtheroid...

Characterization of several high-affinity murine monoclonal anticarcinoembryonic antigen (CEA) antibodies suggested good specificity except for cross-reactivity with an antigen on granulocytes and erythrocytes which was different from the previously described normal cross-reacting antigen of granulocytes. In vivo studies in athymic mice using an indium conjugate of an anti-CEA monoclonal antibo...

The effect of highly purified rheumatoid factor on the precipitin reactions of various antigen-antibody systems was determined. The amount of nitrogen precipitated was increased over a broad range when the factor was added to ovalbumin, human albumin, or human gamma globulin, and the corresponding rabbit antibodies. In the zone of antigen excess, soluble antigen-antibody complexes were precipit...

An electrophoretic technique was combined with an enzyme-linked immunosorbent assay (ELISA) system to achieve a rapid and sensitive immunoassay. A cellulose acetate filter modified with polyelectrolyte multilayer (PEM) was used as a solid substrate for three-dimensional antigen-antibody reactions. A dual electrophoresis process was used to induce directional migration and local condensation of ...

Certain of the serological factors which vary with the individual have been reported,t together with data supporting the idea that age, sex, and blood group differences show some correlation with the antibody titers measured. This supplementary note deals with one of the antigen-antibody systems included in the former study, the essential feature here being the significance of the host origin o...

Ki-67 is a commercially available mouse monoclonal antibody (MoAb), which reacts with a nucleolar antigen (the Ki-67 antigen) expressed in proliferating eukaryotic cells. The author examined the precise localization of the Ki-67 antigen in C-6 cells using immunohistochemical and immunoelectron microscopic methods and estimated the proliferative activity of human brain tumors in situ. Positive n...

The cellular reactions in antibody formation have been studied intensively in recent years. It is now firmly established that antigenic stimulation triggers a typical and specific cellular response in lymph nodes, spleen, and bone marrow, which is characterized by the appearance of hemocytoblasts and the development of immature and mature plasma ceils (1). These cell types are the main contribu...

Sera of 106 normal adult men were tested for antibodies reacting with cell surface antigens of three established lines of cultured malignant melanoma. Positive reactions with a protein A assay for IgG antibodies were extremely rare (1-2%). The frequency of positive reactions with assays for IgM antibodies was higher: 5-15% in immune adherence assays and 55-82% in anti-C3 mixed hemadsorption ass...