We report here on the existence of a new gene for lysine decarboxylase in Escherichia coli K-12. The hybridization experiments with a cadA probe at low stringency showed that the homologous region of cadA was located in lambda Kohara phage clone 6F5 at 4.7 min on the E. coli chromosome. We cloned the 5.0-kb HindIII fragment of this phage clone and sequenced the homologous region of cadA. This r...

Stiff Person Syndrome (SPS) is a rare autoimmune neurological disease attributable to autoantibodies to glutamic acid decarboxylase (anti-GAD) more usually associated with the islet beta cell destruction of autoimmune type 1 diabetes (T1D). SPS is characterized by interference in neurons with the synthesis/activity of the inhibitory neurotransmitter gamma amino butyric acid (GABA) resulting in ...

A commercial hydrolysate of casein stimulated production of lysine decarboxylase (EC 4.1.1.18) by Escherichia coli B. Cellulose and gel chromatography of this hydrolysate yielded peptides which were variably effective in this stimulation. Replacement of individual, stimulatory peptides by equivalent amino acids duplicated the enzyme levels attained with those peptides. There was no indication o...

Mouse brain glutamate decarboxylase(s) was specifically titrated in vivo and in crude brain homogenates by a combination of gabaculine and [a-'H]acetylenic yaminobutyric acid. This specific titration is based on the differential spectra of action of these two mechanism-based enzyme inactivators. The specificity of the titration in vitro was demonstrated by showing that the time course of radioa...

The g-aminobutyric acid (GABA) is a product of decarboxylation of the amino acid glutamate mediated by the synthesizing enzyme glutamic acid decarboxylase (GAD) (1–3). Although GABA is a major inhibitory neurotransmitter of the brain, it is produced at high levels in pancreatic islets (4). b-Cells store GABA in synaptic-like microvesicles, and upon its secretion, GABA exerts many paracrine func...

Objective To describe the retinal nerve fiber layer (RNFL) with demographic and clinical profile in patients glutamic acid decarboxylase 65 (GAD65) glycine receptor (GlyR) neurological autoimmunity. Background GAD65 GlyR autoimmunity can cause a wide range of phenomena, including stiff-person spectrum disorder (SPSD) epilepsy. Both GAD65, through γ-aminobutyric acid-ergic neurons, interact reti...

objective: the aim of the present study was to elucidate the possible biochemical improving effect in lipid metabolic that may result from continuous treatment with green tea extract in normal albino rats and those rendered hyperlipidemic by long term supplementation of fat-enriched diet. materials and methods: fifty male albino rats aged six weeks with 200±10 g weight were randomly divided int...

background: food borne pathogens are of the main concerns of food producers and consumers and escherichia coli and staphylococcus aureus create a lot of problems worldwide. the aim of this study was to evaluate antibacterial effects of monolaurin, sorbic acid and potassium sorbate on s. aureus and e. coli at different ph values and nacl concentrations. methods: micro-well dilution assay was use...

We have cloned and sequenced a full-length cDNA coding for human uroporphyrinogen decarboxylase. The deduced 367-amino acid sequence is consistent with the molecular weight, the partial amino acid sequence of cyanogen bromide peptides, and the total amino acid composition of the purified enzyme. Southern analysis of human genomic DNA shows that its gene is present as a single copy in the human ...