PURPOSE Despite the strong association between malignant disease and thromboembolic disorders, the molecular and cellular basis of this relationship remains uncertain. We evaluated the hypothesis that tumor-derived tissue factor-bearing microparticles in plasma contribute to cancer-associated thrombosis. EXPERIMENTAL DESIGN We developed impedance-based flow cytometry to detect, quantitate, an...

Horizontal information transfer between cells via microparticles is a newly identified communication system. MicroRNAs regulate gene expression and are detected in microparticles. Cantaluppi et al. suggest that microparticles derived from circulating angiogenic cells--'endothelial progenitor cells' (EPCs)--harbor endothelial-protective miRNAs such as miR-126 and that delivery of EPC-derived mic...

       The purpose of this study was to investigate the effect of additives, poly(ethylene glycol) (PEG) 1450, poloxamer 407, polyvinyl alcohol (PVA) and sodium chloride in order to improve physico-chemical characteristics, encapsulation efficiency and in vitro release of bovine serum albumin, form poly(D,L-lactic-co-glycolic acid) (PLGA) microparticles prepared by the w/o/w solvent evaporation...

The transition from invertebrate calcium carbonate-based calcite and aragonite exo- and endoskeletons to the calcium phosphate-based vertebrate backbones and jaws composed of microscopic hydroxyapatite crystals is one of the great revolutions in the evolution of terrestrial organisms. To identify potential factors that might have played a role in such a transition, three key domains of the vert...

Elevated levels of erythrocyte-derived microparticles are present in the circulation in medical conditions affecting the red blood cells. Erythrocyte-derived microparticles expose phosphatidylserine thus providing a suitable surface for procoagulant reactions leading to thrombin formation via the tenase and prothrombinase complexes. Patients with elevated levels of circulating erythrocyte-deriv...

BACKGROUND During storage, red blood cells (RBC) undergo chemical and biochemical changes referred to as "storage lesions". These events determine the loss of RBC integrity, resulting in lysis and release of microparticles. There is growing evidence of the clinical importance of microparticles and their role in blood transfusion-related side effects and pathogen transmission. Flow cytometry is ...

INTRODUCTION In the present study, we investigated the ability of microparticles isolated from synovial fluids from patients with rheumatoid arthritis or osteoarthritis to induce the synthesis and release of key cytokines of B-lymphocyte modulation such as B cell-activating factor, thymic stroma lymphopoietin, and secretory leukocyte protease inhibitor by rheumatoid fibroblast-like synoviocytes...

Biodegradable polylactide microparticles with encapsulated cytotoxic protein viscumin were obtained via the ultrasound-assisted supercritical fluid technique. The size of the microparticles was 10-50 µM, as shown by electron microscopy. The time course of viscumin release from microparticles was studied using an immunoenzyme test system with anti-viscumin monoclonal antibodies. It was found tha...

The influence of morphology and surface properties on the therapeutic efficacy of degradable polymeric microparticles has not been well understood. One of the primary reasons for this is the limited ability to fabricate microparticles with controlled morphology and surface properties. Here, we report the electrospraying of blends of Pluronic(®) with poly(lactide-co-glycolide) (PLGA) as a novel,...

Microparticles of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(ε-caprolactone) (PCL) containing resveratrol were successfully prepared by simple emulsion/solvent evaporation. All formulations showed suitable encapsulation efficiency values higher than 80%. PHBV microparticles revealed spherical shape with rough surface and presence of pores. PCL microparticles were spherically s...