The methods of extraction, separation and analysis of alkaloids and indole glucosinolates (GLs) ofIsatis tinctoria were reviewed. Different analytical approaches such as High-pressure Liquid Chromatography (HPLC), Liquid Chromatography with Electrospray Ionization Mass Spectrometry (LC/ESI/MS), Electrospray Ionization Time-Of-Flight Mass Spectrometry (ESI-TOF-MS), and Nuclear Magnetic Resonance...

Nifedipine and NifedipineD6, (IS) were extracted from the plasma by precipitation method and then separated on a Reverse phase chromatography using the mobile phase mixture of ammonium acetate and methanol at a flow rate of 1.0 ml/min. The analytes were detected in API 4000 Mass spectrometer in the positive atmospheric pressure chemical Ionization (APCI) mode with multiple reactions monitoring ...

A liquid chromatography-tandem mass spectrometry method with atmospheric pressure chemical ionization (LC-APCI/MS/MS) was validated for the determination of etoricoxib in human plasma using antipyrin as internal standard, followed by on-line solidphase extraction. The method was performed on a Luna C 18 column and the mobile phase consisted of acetonitrile:water (95:5, v/ v)/ammonium acetate (p...

You, J., X. Zhao, Y. Suo, Y. Li, H. Wang, and G. Chen, Determination of LongChain Fatty Acids in Bryophyte Plant Extracts by HPLC with Fluorescence Detection and Identification with MS, J. Chromatogr. B 848:283–291, 2007. A sensitive method has been developed for the determination of long-chain fatty acids (LCFAs) (>C20) using 1-[2-(ptoluenesulfonate)-ethyl]-2-phenylimidazole-[4,5-f]-9,10-phena...

It has previously been demonstrated that 2,4,6-trinitrotoluene (TNT) exhibits characteristic reactivity with negative reactant ions generated in air or O2 during atmospheric-pressure chemical ionization (APCI). For instance, the reaction products were TNT? • (m/z 227) for O2? •, [TNT?H•]? 226) NO3?, and [TNT ? OH•]? 210) NO•]? 197) O3? •. In this work, reactions of TNT atmospheric pressure CO2 ...

The recent discovery in hops of trans-resveratrol and its glucoside, trans-piceid, both famous in wine for their potential role in the "French paradox", opens new doors to understanding hop health benefits. In the present work, trans-resveratrol and trans-piceid were quantified by HPLC-APCI-MS/MS in 40 American and European hop cone samples from harvests of 2004, 2005, and 2006. Their content v...

EPA Method 8330B for trace explosives in water by HPLC was transferred from UV detection to mass spectrometry detection using the Agilent 1290 Infinity LC System coupled to the Agilent 6460 Triple Quadrupole LC/MS System using APCI negative mode. Eleven explosive compounds were analyzed with this method at trace concentrations in water samples with limits of detection at the 5–50 ng/L concentra...

H and C NMR spectra were recorded with Bruker DPX-400, AVANCE-400 and DRX-500. The numbering of the protons relate to the numbering chosen in Fig. 1. High resolution mass spectra were obtained with a Micromass LCT via loop-mode injection from a Waters (Alliance 2695) HPLC system. Alternatively a Micromass Q-TOF in combination with a Waters Aquity Ultraperformance LC system was employed. Ionizat...