signaling to orchestrate cell shape changes during mesoderm invagination. The apically targeted transmembrane protein T48 acts as an anchor for RhoGEF2 via a PDZ binding motif and thereby ensures the apical enrichment of RhoGEF2 during mesoderm invagination allowing rapid and efficient ventral furrow formation. Apical constriction also requires the tethering of the contractile network to the ad...

The absence of a natural apical constriction in permanent tooth makes endodontic treatment a challenge. Traditionally, calcium hydroxide was used for inducing the formation of an apical barrier. Currently, mineral trioxide aggregate (MTA) has shown promising results for apexification procedures. In an open apex, it is imperative to limit the MTA placement within the confines of the root canal f...

Tissue elongation and its control by spatiotemporal signals is a major developmental question. Currently, it is thought that Drosophila ovarian follicular epithelium elongation requires the planar polarization of the basal domain cytoskeleton and of the extra-cellular matrix, associated with a dynamic process of rotation around the anteroposterior axis. Here we show, by careful kinetic analysis...

signaling to orchestrate cell shape changes during mesoderm invagination. The apically targeted transmembrane protein T48 acts as an anchor for RhoGEF2 via a PDZ binding motif and thereby ensures the apical enrichment of RhoGEF2 during mesoderm invagination allowing rapid and efficient ventral furrow formation. Apical constriction also requires the tethering of the contractile network to the ad...

Remodeling of epithelial sheets plays important roles in animal morphogenesis. Shroom3 is known to regulate the apical constriction of epithelial cells. Here, we show that Shroom3 binds ROCKs and recruits them to the epithelial apical junctions. We identified the Shroom3-binding site (RII-C1) on ROCKs, and found that RII-C1 could antagonize the Shroom3-ROCK interaction, interfering with the act...

Objective: Root canal anatomy significantly impacts the working length determination. This study aimed to investigate effect of preflaring technique on accuracy four electronic apex locators. Methods: Forty-five single-canal central maxillary teeth were selected. The was measured before and after coronal preparation by types locators in range ±0.5 ±1 mm matched for apical constriction. actual c...

This study evaluated the accuracy of three electronic apex locators (Root ZX, Novapex, and Justy II) in root canal length determinations using different apical file sizes, considering the apical constriction (AC) and the major foramen (MF) as anatomic references. The diameter of the apical foramina of 40 single-rooted teeth was determined by direct visual measurement and the master apical file ...

Apical constriction During several morphogenetic developmental processes, epithelial sheets contract some of their cells to cause invaginations or bending. To do this, epithelial cells constrict apically located actin and myosin while lengthening the basolateral axis in a process called apical constriction. Frank Mason (Martin Laboratory, Massachusetts Institute of Technology) showed that RhoA ...

Taurodontism is a morpho-anatomical developmental anomaly, which is seen infrequently in teeth only. It is characterized by a deficiency in the constriction at the cement-enamel junction, with lengthened pulp chamber and apical displacement of the pulpal floor. This report presents a case of bilateral taurodontism which were pulpally involved. Endodontic treatment was done for both the teeth an...