Background: Cancer refers to the abnormal growth of cells and is still the most common cause of morbidity in world. The purpose of this study was to determine cytotoxicity effect of high molecular weight (HMWC) and low molecular weight of chitosan (LMWC) on three cancerous cell lines MCF-7, HeLa and Saos-2 with different histological origin. Methods: The anticancer property of two types of chi...

v Apoptosis can be induced in several cell lines by photodynamic treatment with different photosensitizers including aminolevulinic acid (ALA)-stimulated endogenous protoporphyrin IX (PpIX). This PpIX is successfully used as tumor marker in diagnosis, and owns good sensitizing properties for tumor therapy (PDT). The aim of the study was to investigate cell death mechanisms and its consequences ...

background: adipose-derived stem cells (adscs) are a population of pluripotent cells used for tissue engineering purposes. the main purpose of the present study was to transdifferentiate the adscs to schwann-like cells and to determine the intensity of apoptosis in adscs during the transdifferentiation process.   methods: adscs were isolated from the inguinal adipose tissue of adult rats and th...

Isochlorogenic acid C (ICAC) is found in a variety of natural foods and medicinal plant materials. This study aims to validate the biological activity isochlorogenic limiting inflammation by Erk/JNK/NF-κB pathway. In this study, TNF-α-induced human fibroblast-like synoviocytes collagen-induced arthritis animal models were used perceive potential anti-inflammation mechanism ICAC. The role was ev...

Endoplasmic reticulum (ER) stress can induce autophagy via the unfolded protein response (UPR), and regulate activation of inflammasomes. Inositol-requiring enzyme 1α (IRE1α) is a transducer UPR in cells with ER stress. Here, we investigated role IRE1α its impact on rheumatoid arthritis fibroblast-like synovial (RA-FLSs). RA-FLSs were isolated from (RA) patients stimulated thapsigargin (TG) to ...

The photophysics of hypericin have been studied in its complex with two different isoforms, A1-1 and P1-1, of the protein glutathione S-transferase (GST). One molecule of hypericin binds to each of the two GST subunits. Comparisons are made with our previous results for the hypericin/human serum albumin complex (Photochem. Photobiol. 1999, 69, 633-645). Hypericin binds with high affinity to the...

Evaluation of in-vitro apoptosis induction, cytotoxic activity Hymenodictyon excelsum (Roxb) Wall in Dalton’s lymphoma ascites (DLA) and Lung fibroblast - Mouse L929 cell linesKalathil Khairunnisa Dhananjayan Karthik

Hypericum perforatum is well known for its antidepressant and anti-inflammatory activities, for which hypericin and its derivatives are indicated to be the most active compounds. Hypericin synthase (Hyp-1) is the only protein proven to catalyze the synthesis of hypericin. In this study, the full-length cDNA of Hyp-1 was chemically synthesized according to the Hyp-1 sequence in GenBank (accessio...

It has been reported that Notch family proteins are expressed in synovium tissue and involved in the proliferation of synoviocyte from rheumatoid arthritis (RA). The aim of this paper was to investigate whether Notch signaling mediated TNF-α-induced cytokine production of cultured fibroblast-like synoviocytes (FLSs) from RA. Exposure of RA FLSs to TNF-α (10 ng/ml) led to increase of Hes-1, a ta...