The yeast strain (Saccharomyces cerevisiae) MTCC 3157 was selected for combinatorial biosynthesis of plant sesquiterpene amorpha-4,11-diene. Our main objective was to overproduce amorpha 4-11-diene, which is a key precursor molecule of artemisinin (antimalarial drug) produced naturally in plant Artemisia annua through mevalonate pathway. Farnesyl diphosphate (FPP) is a common intermediate metab...

Most TPSs (terpene synthases) contain plasticity residues that are responsible for diversified terpene products and functional evolution, which provide a potential for improving catalytic efficiency. Artemisinin, a sesquiterpene lactone from Artemisia annua L., is widely used for malaria treatment and progress has been made in engineering the production of artemisinin or its precursors. In the ...

Drugs are primary weapons for reducing malaria in human populations. However emergence of resistant parasites has repeatedly curtailed the lifespan of each drug that is developed and deployed. Currently the most effective anti-malarial is artemisinin, which is extracted from the leaves of Artemisia annua. Due to poor pharmacokinetic properties and prudent efforts to curtail resistance to monoth...

BACKGROUND AND THE PURPOSE OF THE STUDY Artemisinin is one of the most effective medicine against malaria, which is produced naturally by Artemisia annua in low yield. It is produced in a metabolic pathway, in which several genes and gene products are involved. One of the key genes in this pathway is am1, which encodes amorpha-4, 11-diene synthase (ADS), a key enzyme in artemisinin biosynthesis...

--The inhibition of the corrosion of mild steel in hydrochloric acid solutions by the extract of Artemisia Herba-Alba has been studied using gravimetric and electrochemical measurement. Inhibition was found to increase with increasing concentration of the inhibitor. Values of inhibition efficiency calculated from weight loss and potentiodynamic polarization are in good agreement. This value goe...

There is increasing resistance of malaria parasites to chloroquine, the cheapest and commonly used drug for malaria in Nigeria. Artemisin, a product from medicinal plant indigenous to China, based on active principle of Artemisia annua, has been introduced into the Nigerian market. However not much has been done to project antimalaria properties of indigenous medicinal plants. This study thus, ...

Artemisinin is a sesquiterpenoid lactone peroxide, known for its potent antimalarial activity that can be extracted from Artemisia annua L. This compound only sparingly soluble in water, making extraction using environmental-friendly and non-toxic aqueous solvents difficult. In the attempt to overcome this limitation, hydrotropes, which are class of compounds assist increasing solubility hydrop...

Abstract The leaves of different species the genus Artemisia show considerable inner and external structural diversity. This paper presents a comparative study micromorphological features leaf surface for seven from regions Ukraine: A. absinthium L., annua dracunculus marschalliana Spreng., vulgaris argyi H.Lév. & Vaniot, verlotiorum Lamotte. vegetative organs these plants have potentially ...

Objective: To evaluate the anthelmintic activity of Artemisia indica. Methods: In the present investigation, the in vitro anthelminthic activity of chloroform, methanol and aqueous extracts of Artemisia indica was carried out against Indian adult earthworm Pheretima posthuma. Piperazine citrate was used as the standard reference. Five different concentrations (1.25, 2.5, 3.75, 5.0 and 6.25 mg/m...