Herein, we studied the impact of surface roughness on molecular dynamics a series phenyl-terminated monohydroxyalcohols under spatial confinement provided by nanoporous anodic aluminum oxide (AAO) membranes constant (const-AAO) and modulated (modul-AAO) pore diameter using Broadband Dielectric Spectroscopy Differential Scanning Calorimetry. Interestingly, observed that both types AAO affect beh...

showed no respiration due to endogenous substrates. Only L-ascorbate with NNN‘N’tetramethyl-p-phenylenediamine was oxidized by membrane particles and the specific activity obtained was lower than in corresponding particles prepared from NH4+-grown cells. Low-temperature reduced-minus-oxidized difference spectra (Fig. 1, trace b) suggested that this lack of electron-transport activity may be due...

Though there are undoubtedly multiple functions for glutathione yet to be appreciated we do know that glutathione is: • a co-factor for the glutathione peroxidases, which are crucial selenium-containing antioxidant enzymes. • a co-factor for glutathione S-transferases, enzymes which are involved in the detoxification of xenobiotics, including carcinogens. • involved in the regeneration of ascor...

Recycling of ascorbic acid from its oxidized forms is required to maintain intracellular stores of the vitamin in most cells. Since the ubiquitous selenoenzyme thioredoxin reductase can recycle dehydroascorbic acid to ascorbate, we investigated the possibility that the enzyme can also reduce the one-electron-oxidized ascorbyl free radical to ascorbate. Purified rat liver thioredoxin reductase c...

Low millimolar concentrations of ascorbate are capable of inflicting lethal damage on a high proportion of cancer cells lines, yet leave non-transformed cell lines unscathed. Extracellular generation of hydrogen peroxide, reflecting reduction of molecular oxygen by ascorbate, has been shown to mediate this effect. Although some cancer cell lines express low catalase activity, this cannot fully ...

Copper oxide (CuO) nanotubes were successfully converted from CuS nanowires embedded in anodic aluminum oxide (AAO) template by annealing-induced diffusion in a confined tube-type space. The spreading of CuO and formation of CuO layer on the nanochannel surface of AAO, and the confinement offered by AAO nanochannels play a key role in the formation of CuO nanotubes.

d-Serine normally contributes up to 3% to total plasma serine and up to 23% in chronic renal failure. d-Serine is metabolized by tubular d-amino acid oxidase (D-AAO), and highd-serine plasma levels are nephrotoxic; both events are localized in the straight part of the proximal tubule. We therefore investigated if and howd-serine is reabsorbed there. We microinfused14C-labeledd- or -l-serine + [...

Substrate kinetic properties of cytochrome oxidase in rat liver, kidney, brain and heart mitochondria were examined using ascorbate + N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) as the electron donor system. Analysis of the substrate kinetics data revealed tissue-specific expression of kinetic components exhibiting differences with respect to Km, Vmax and Kcat/Km values. Regression analysis...

clinical symptoms of all MC were in line with classical PD. For comparison of AAO data, we used published AAO of a large population-based cohort. The AAO of 197 idiopathic PD patients was 70 6 11 years, whereas the AAO of MC with one mutation in our meta-analysis was 42 6 16 years (P < 0.0001). It should be noted however, patients with young onset of PD are more likely to be included in genetic...