Ascorbic acid donates electrons to dopamine beta-monooxygenase during the hydroxylation of dopamine to norepinephrine in vitro. However, the possible role of ascorbic acid in norepinephrine biosynthesis in vivo has not been defined. We therefore investigated the effect of newly accumulated ascorbic acid on catecholamine biosynthesis in cultured bovine adrenal chromaffin cells. Cells supplemente...

CONTEXT Some animal studies suggest that orally administered ascorbic acid may chelate lead and decrease the risk of the toxic effects of lead. However, results from several small studies in humans have yielded inconclusive evidence of a beneficial effect of ascorbic acid on lead toxicity. OBJECTIVE To examine the relationship between serum ascorbic acid levels and prevalence of elevated bloo...

The ATDC5 cell line exhibits a multistep process of chondrogenic differentiation analogous to that observed during endochondral bone formation. Previous investigators have induced ATDC5 cells to differentiate by exposing them to insulin at high concentrations. We have observed spontaneous differentiation of ATDC5 cells maintained in ascorbic acid-containing alpha-MEM. A comparison of the differ...

1. No difference in health was observed between men on megadosage of ascorbic acid and controls during the year of an Antarctic expedition. 2. All men appeared to have a satisfactory intake of ascorbic acid throughout the year. 3. There was a statistically significant decrease in excretion of ascorbic acid by men on megadosage over the year, and by the controls. 4. The decline in excretion by t...

25 The ATDC5 cell line exhibits a multistep process of chondrogenic differentiation analogous to 26 that observed during endochondral bone formation. Previous investigators have induced 27 ATDC5 cells to differentiate by exposing them to insulin at high concentrations. We have 28 observed spontaneous differentiation of ATDC5 cells maintained in ascorbic acid-containing 29 αMEM. A comparison of ...

Both ascorbic acid and copper were strong prooxidants in the oxidation of linoleate in a buffered (pH 7.0) aqueous dispersion at 37OC. Minimum concentrations at which catalytic activity was detected were 1.3 X 10-7 M for coppcr and 1.8 X 10-6 M for ascorbic acid. For concentrations up to lop3 M, the increase in rate of oxidation with increase in concentration of catalyst was greater for ascorbi...

turmeric (cucurma longa) has been used as an antioxidant agent in medical as well as health sciences some years ago. however, it has not been applied in tissue culture till now. browning tissues is result of tissue damages caused by polyphenoloxidase enzyme. in order to study this problem and reduce browning, explants are cultured in culture media containing antioxidants including citric acid, ...

BACKGROUND Concern has been raised that high levels of ascorbic acid consumption may lead to potential adverse effects, such as vitamin B12 deficiency, iron overload, and kidney stones. OBJECTIVE To examine the relation of serum ascorbic acid level, which reflects intake, to serum vitamin B12 level, serum ferritin level, and kidney stones. METHODS We analyzed data collected on a random samp...

1. The effect ofdietary molybdenum on the growth rate and also on ascorbic acid metabolism in rats was studied. An excess of dietary molybdenum resulted in growth retardation and loss of weight. Tolerance to molybdenum was affected by the nature ofthe molybdenum salt administered. 2. Molybdenum ingestion altered certain aspects of ascorbic acid metabolism in rats. The conversion ofD-glucuronola...