Proteins are often separated using ion-exchange chromatography. This method can however show poor resolution if not carefully optimised. This work aims to find all the parameters that affect the separation as well as determining which are critical. By using Design of Experiment the optimum separation is found and a robustness analysis is conducted. Polyclonal antibody Immunoglobulin G (IgG) and...

The colloidal dye lithium carmine was added in vitro to normal human serum. Electrophoretic experiments showed that the dye was associated mainly with a2-globulins, small amounts with the albumin and only traces with the y-globulins. The main complex was eluted with the macroglobu­ lin peak obtained by gel filtration on Sephadex G-200 and sedimented in the heavy fraction on density gradient ult...

The application of the phenol-duponol method to extraction of nucleic acids from HeLa cells is described. Chromatography of the phenol extract on an esterified bovine serum albumin column with a salt gradient of sodium chloride gives separation of soluble RNA, DNA, and two different high molecular RNA fractions. Ultracentrifugation of the DNA eluted from the column gives a sedimentation coeffic...

The functional attributes of minor subpopulations of mouse thymus cells derived by bovine serum albumin density gradient centrifugation, cortisone treatment, or selective depletion by anti-TL or anti-theta treatment have been examined. A subpopulation derived in each fashion contains the cells required to evoke graft-versus-host reactions in neonatal F(1) hybrid recipients and to be stimulated ...

background:extrauterine growth restriction remains a common and serious problem in newborns especially who are small, immature, and critically ill. very low birth weight infants (vlbw) had 97% and 40% growth failure at 36 weeks and 18-22 months post-conceptual age respectively. the postnatal development of premature infants is critically dependent on an adequate nutritional intake that mimics a...

A man diagnosed with alcoholic liver cirrhosis complained of abdominal distention due to massive ascites. The ascites did not resolve with diuretic agents. The serum-ascites albumin gradient value of 1.9 g/dL and the total protein level in the ascites of 3.1 g/dL indicated the ascites to have been caused by portal hypertension. Hypothyroidism was detected, and the patient received supplementati...

A dose-related inhibition by halothane of adenosine diphosphate-induced platelet aggregation was measured for human platelets in plasma anticoagulated with sodium citrate 6.8 mmol litre-1 or 13.6 mmol litre-1, or heparin 2 u. ml-1 and for platelets separated from plasma by albumin density gradient centrifugation and requiring no anticoagulant. By maintaining exposure and assay temperature at 37...

Background. Intraabdominal lesions such as mesenteric cysts are uncommon disorders. Most are discovered incidentally during routine abdominal examinations. Methods. We report a patient with a mesenteric cyst masquerading as tuberculous peritonitis and ascites. Conclusion. Mesenteric cysts generally do not show typical clinical findings. They may also present with peritoneal tuberculosis finding...

Serum Albumin is the most aboundant protein in blood plasma. Its two major roles aremaintaining osmotic pressure and depositing and transporting compounds. In this paper,Albumin-methanol solution simulation is carried out by three techniques including MonteCarlo (MC), Molecular Dynamic (MD) and Langevin Dynamic (LD) simulations. Byinvestigating energy changes by time and temperature (between 27...

Although the factors involved in cirrhotic ascites have been studied for a century, a number of observations are not understood, including the action of diuretics in the treatment of ascites and the ability of the plasma-ascitic albumin gradient to diagnose portal hypertension. This communication presents an explanation of ascites based solely on pathophysiological alterations within the perito...