Abstract This study investigated the effect of oleo-gum-resin from Commiphora molmol Engler (Family: Burseraceae), commonly known as myrrh and as the commercial extract Mirazid, as a treatment for hepatic coccidiosis induced by the parasite Eimeria stiedae in domestic rabbits. Rabbits (Oryctolagus domesticus L.) were infected with sporulated parasite oocysts and subjected to treatment regimens ...

A hitherto unknown cytosolic glutathione S-transferase from rat liver was discovered and a method developed for its purification to apparent homogeneity. This enzyme had several properties that distinguished it from other glutathione S-transferases, and it was named glutathione S-transferase X. The purification procedure involved DEAE-cellulose chromatography, (NH4)2SO4 precipitation, affinity ...

In the present study, changes of values of serum 17ß-estradiol (E2), Asparate amino teransferase (AST), Alanine amino transferase (ALT) and Lactate dehydrogenase (LDH) following intraperitoneal (i.p) injection with the aromatase inhibitor (AI) letrozole were investigated in rainbow trout (Oncorhynchus mykiss). Forty- eight apparently healthy rainbow trout (mean weight of 845 g) divided...

BACKGROUND Changes in the activities of plasma alanine amino transferase (ALT), aspartate amino transferase (AST), gamma glutamyl transferase (GGT), and alkaline phosphatase (ALP) are used to assess the functional state of the liver. Significant increase in the activities of these enzymes commonly indicates the hepatotoxicity of chemical agent(s) in the body. Exposure of male and female rats to...

Background To explore the antioxidant and hepatoprotective effect of ethanolic Mangifera indica (EMI) and methanolic Mangifera indica (MMI) leaf extracts in mercuric chloride (HgCl2) induced toxicity in Swiss albino mice. Materials and methods Toxicity in mice was induced with HgCl2 (5.0 mg/kg, i.p.), followed by oral intervention with EMI and MMI extracts (25 mg and 50 mg/kg. body wt.) for 3...

OBJECTIVE Comparison of gamma glutamyltransferase in normal and type 2 diabetics. METHODS In a cross-sectional study, 100 apparently normal healthy subjects and, 47 type 2 diabetic subjects were selected from either sex with ages between 18-65 years. Subjects were measured for waist/hip ratio, BMI and serum levels of ALT, AST, Alk phosphatase and glutamyl transferase (GGT). The study excluded...

Apigenin, a dietary flavonoid, is reported to have several therapeutic effects in different diseases including cancer. Toxicity of Apigenin is however, least explored, and reports are scanty in literature. This warrants dose-specific evaluation of toxicity in vivo. In the present study, Apigenin was administered intraperitoneally to Swiss mice at doses of 25, 50, 100 and 200 mg/kg. Serum levels...

N,N-Dimethylformamide (DMF), an industrial solvent widely used throughout the world is a known toxic compound. Here, we studied the effects of acute exposure of DMF on liver and kidney in rats. Rats were treated intraperitoneally with a single dose of DMF (1.5 g/kg) for 24 and 48 h. Hepatic and nephrotoxicity was confirmed based on the significant increase in the serum levels of aspartate amino...

The alpha-L-fucosidase from Thermotoga maritima (Tm alpha fuc) was converted into alpha-L-transfucosidase variants by directed evolution. The wild-type enzyme catalyzes oligosaccharide synthesis by transfer of a fucosyl residue from a pNP-fucoside donor to pNP-fucoside (self-condensation) with alpha-(1-->3) regioselectivity or pNP-galactoside (transglycosylation) with alpha-(1-->2) regioselecti...

The mouse mammary tumor virus (MMTV) protease gene was cloned into pGEX-QT, an Escherichia coli expression vector containing the glutathione S-transferase coding region of Schistosoma japonicum. The chimeric protein was formed by fusion of the glutathione S-transferase with a hexapeptide which contains a thrombin cleavage site, followed by the MMTV protease. Affinity chromatography on a glutath...