Since the advent of a novel technique of enzyme resolution of bacterial aspartic acid deaminase (Lichstein and Umbreit, 1947), this enzyme has been linked with biotin (Liclstein and Umbreit, 1947; Wright et al., 1949), with adenylic acid (Lichstein and Christman, 1948), and with a coenzyme present in yeast and liver extracts (Lichstein and Christman, 1949; Christman and Lichstein, 1950). These ...

Two radioactive glycopeptides (Glycopeptides I and II) were isolated from the deoxycholate-soluble fraction of rat liver microsomes 90 min after injection of glucosamine-l-14C. These two glycopeptides were rich in mannose and N-acetylglucosamine but relatively poor in galactose and sialic acid. Glycopeptide I contained sialic acid, galactose, N-acetylglucosamine, mannose, aspartic acid, serine,...

Aminophosphonic acids analogous to glutamic acid, aspartic acid, alanine, and valine were actively accumulated by Lactobacillus plantarum. Uptake was dependent on the availability of glucose and, in all cases, the estimated intracellular concentrations substantially exceeded extracellular levels. During uptake, there was little metabolism of tritiated 2-amino-3-phosphonopropionic acid (APP), th...

The data provide information in support of the research article, "The cleavage specificity of the aspartic protease of cocoa beans involved in the generation of the cocoa-specific aroma precursors" (Janek et al., 2016) [1]. Three different protein substrates were partially digested with the aspartic protease isolated from cocoa beans and commercial pepsin, respectively. The obtained peptide fra...

A fast perfusion system was used to apply excitatory amino acids to embryonic hippocampal neurons grown in dissociated culture and voltage clamped in the whole-cell recording configuration. Responses to quisqualic acid and DL-alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA; a potent quisqualate-like agonist) showed rapid desensitization: at 100 microM the peak inward current dec...

Strong evidence indicates that virions of mammalian reoviruses undergo proteolytic processing by acid-dependent cellular proteinases as an essential step in productive infection. Proteolytic processing takes the form of a series of cleavages of outer-capsid proteins final sigma3 and mu1/mu1C. Previous studies showed an effect of both NH4Cl and E-64 on these cleavages, indicating that one or mor...