To investigate the changes in transcript and relative protein levels in response to temperature, complementary transcriptomic and proteomic analyses were used to identify changes in Aspergillus flavus grown at 28 °C and 37 °C. A total of 3,886 proteins were identified, and 2,832 proteins were reliably quantified. A subset of 664 proteins was differentially expressed upon temperature changes and...

Aspergillus flavus is a soil fungus that commonly invades peanut seeds and often produces carcinogenic aflatoxins. Under favorable conditions, the fungus-challenged peanut plant produces and accumulates resveratrol and its prenylated derivatives in response to such an invasion. These prenylated stilbenoids are considered peanut antifungal phytoalexins. However, the mechanism of peanut-fungus in...

Antimicrobial activity of 25, 50, 75 and 100% alcohol extract of Piper nigrum (Black Pepper), Psidium guajava (Guava), Amomum cardamom (Cardamom) and Cynogen dactylon (Bermuda grass) has been evaluated against Pseudomonas lundensis, Bacillus cereus, Aspergillus niger and Aspergillus flavus. Psidium guajava extract showed excellent antimicrobial activity against all the test organisms. In 25% co...

Racemic, (−)-, and (+)-gossypol were evaluated over a concentration range of 0 to 400 μg per mL for effects on several Aspergillus flavus growth parameters. In the concentration range tested, racemic gossypol did not stimulate conidial germination on water agar alone. In a germination stimulatory environment (potato dextrose medium), racemic gossypol concentrations of ≥ 200 μg per mL decreased ...

Efficacy of some local plants in the management of Aspergillus flavus and aflatoxin contamination was investigated. Aqueous extracts of rhizome of Zingiber officinalis and leaf extracts of Trigonella foenum-graecum inhibited aflatoxin production to a significant level. While solvent extracts of Zingiber officinalis and Oxalis corniculata could suppress both the growth and aflatoxin production b...

Xanthobacter flavus, a gram-negative facultatively autotrophic bacterium, employs the Calvin cycle for the fixation of carbon dioxide. Cells grown under autotrophic growth conditions possess an Fe(2+)-dependent fructosebisphosphate (FBP) aldolase (class II) in addition to a class I FBP aldolase. By nucleotide sequencing and heterologous expression in Escherichia coli, genes encoding transketola...

Efficacy of three different concentrations (5, 10 and 15 mg/ml) of dry flower powder of E. splendens and L. nepetaefolia was tested on the growth of aflatoxin-producing toxigenic strains of fungi A. flavus (NCBT 101) and A. parasiticus (NCBT 128) in Sabouraud dextrose agar medium (SDA). Maximum (75%) inhibition of growth of A. flavus was seen at 15 mg/ml concentration of E. splendens flower dry...

Aflatoxins are highly carcinogenic compounds produced by the fungus Aspergillus flavus. Aspergillus flavus is a phytopathogenic fungus that commonly infects crops such as cotton, peanuts, and maize. The goal of this research was to design an effective method for sample preparation and analysis of aflatoxin B1 from Aspergillus flavus infected maize kernels. The method involves liquid extraction ...

Aflatoxins are toxic and carcinogenic polyketide metabolites produced by certain fungal species, including Aspergillus flavus and A. parasiticus. Many internal and external factors, such as nutrition and environment affect aflatoxin biosynthesis; therefore, we analyzed the transcriptome of A. flavus using expressed sequence tags (ESTs) from a normalized cDNA expression library constructed from ...