Bacillus represents microbes of high economic, medical and biodefense importance. Bacillus strain identification based on 16S rRNA sequence analyses is invariably limited to species level. Secondly, certain discrepancies exist in the segregation of Bacillus subtilis strains. In the RDP/NCBI databases, out of a total of 2611 individual 16S rDNA sequences belonging to the 175 different species of...

Proteolysis-resistant lipases can be well exploited by industrial processes which employ both lipase and protease as biocatalysts. A proteolysis resistant lipase from Bacillus pumilus SG2 was isolated, purified and characterized earlier. The lipase was resistant to native and commercial proteases. In the present work, we have characterized the lip gene which encodes the proteolysis-resistant li...

New methods and technology are needed to quickly and accurately detect potential biological warfare agents, such as Bacillus anthracis, causal agent of anthrax in humans and animals. Here, we report the detection of a simulant of B. anthracis (B. globigii) alone and in a mixture with a different species of Bacillus to test non-specific interference using a portable surface plasmon resonance (SP...

This study was done to assess the antifungal effect of some biocontrol agents effective microorganisms (EMs1), Pseudomonas fluorescens, and Bacillus pumilus, titanium dioxide (TiO2) nanoparticles, black cumin and wheat germ oils as well as the recommended fungicide (flutolanil) against root rot of tomato. Moreover, gas chromatography-mass spectrometry (GC-MS) examination was completed to identi...

objective(s): we aimed to characterize the phenotype and genotype of bacillus spp isolated from diabetic patients’ eyes, by studying the drug sensitivity patterns with a disc-diffusion method. materials and methods: fifty eyes of 25 patients with type ii diabetes mellitus, with at least 10 years of diabetes history, were included in the study. we analyzed the eyes for the presence of bacillus s...

Abstract One of the mitigations in dealing with problem waste oil is by using bioremediation techniques. Bioremediation techniques use biological agents that are safe for environment and more cost-effective. The method commonly developed immobilization bacteria plant fibers. fiber used ketapang leaf ( Terminalia catappa ) because it has physico-chemical properties can be bioremediation. This st...

The impact of individual and combined pulsed electric field (PEF) high power ultrasound (HPU) on the inactivation different microorganisms in emulsions was investigated. highest level using PEF 2.6, 1.2 0.1 log-cycles for Escherichia coli, Aspergillus niger Bacillus pumilus, respectively, achieved at energy temperature (152.3–176.3 kJ/kg 25 °C). HPU led to reduction (5.4, 4.3 0.3 E. A. B. respe...

The microbial ecology within the rhizosphere of Tylenchulus semipenetrans-infected Citrus L. trees was examined. Sixty bacterial strains were obtained from roots infected and eggs T. semipenetrans. Among these some agar plates two nematophagous fungi, Dactylellina gephyropaga (Drechsler) Ying Yang & Xing Z. Liu Arthrobotrys conoides Drechsler. Bacterial identified using 16S rDNA, gyrA rpoB gene...