Germination of spores of the genus Bacillus can be stimulated by heat, L-alanine, or manganese. The reversal of L-alanine stimulation by D-alanine (Hills, 1950) appears to be specific and, in the present study, affords a tool for the exploration of the mechanisms and interrelations among heat, manganese and L-alanine stimulations. The heat stimulation of bacterial spore germination has long bee...

early in this study, an α-amylase from bacillus megaterium who (bmw) was isolated from hot springs of ramsar (north of iran), and its gene was cloned in e.coli. based on its conserved sequence regions and substrate specificity, it was classified as intermediary group enzymes with the specificity of oligo-1,6-glucosidase and neopullulanase subfamilies. in the sixth conserved region (83-qvngiwmmp...

This study was carried out to investigate the impacts of bacterial inoculation on texturally different soil samples under greenhouse conditions. Sandy loam (SL), clay (CL), and (C) textured soils were inoculated with Bacillus arenosi K64 (B. arenosi), megaterium M3 megaterium), subtilis OSU 142 subtilis) isolates then incubated at 5 periods [I (20 days), II (40 III (60 IV (80 V (100 days)]. Soi...

The stock solution 10% and half 5% of three oils namely; mineral oil (Diver 97 % EC), plant marjoram (Origanum majorana L.) orange (Citrus aurantium L.), as well two species bacteria Bacillus megaterium BTS1 (Bacillus poylmyxa)at concentrations showed that nematicidal activity against egg-mass second stage juveniles Meloidogyne incognitaKofoid in vitro experiments. Egg hatching juvenile mortali...

Bacillus strains have been widely used as the companion strain of Ketogulonigenium vulgare in the process of vitamin C fermentation. Different Bacillus strains generate different effects on the growth of K. vulgare and ultimately influence the productivity. First, we identified that Bacillus endophyticus Hbe603 was an appropriate strain to cooperate with K. vulgare and the product conversion ra...

A simple growth medium is reported, in which Bacillus megaterium forms heat-stable spores, heat-labile spores, or only vegetative cells by changing the carbon and nitrogen source. Studies carried out in such media could be very useful in elucidating biochemical events which lead to bacterial sporulation.

On base of stock-breeding and poultry wastes the cheap nutrient media for cultivation and production of biomass of phototrophic bacteria have been developed. Cultivation of purple nonsulfur bacteria Rhodobacter sphaeroides together with Bacillus megaterium in mixed culture has been succeeded in high biomass yield. The obtained biomass was distinguished with rich contents of biologically active ...

Helium displacement and nitrogen adsorption techniques were used to determine the density and porosity, respectively, of freeze-dried cell walls isolated from Bacillus megaterium KM and Saccharomyces cerevisiae. The densities were 1.302 and 1.180 g/cm3, respectively, suggesting noncrystalline solids. The porosities were extremely small, indicating that the cell walls had collapsed and become es...

Cells of Bacillus megaterium can use the externally added precursors UDP-N-acetyl-D-glucosamine and UDP-N-acetylD mU!‘aIUyl L danyl D ghItamy1 mes o diaminopimelyl D alanyl-n-alanine to synthesize peptidoglycan if the bacteria were first treated with toluene. Toluene-treated bacteria could polymerize these precursors into peptidoglycan which was attached to previously existing cell wall by pept...