This study reports the purification and biochemical characterization of thermostable and acidic-pH-stable α-amylase from Bacillus sp. Iranian S1 isolated from the desert soil (Gandom-e-Beryan in Lut desert, Iran). Amylase production was found to be growth associated. Maximum enzyme production was in exponential phase with activity 2.93 U ml-1 at 50°C and pH 5. The enzyme was purified by isoprop...

The alpha-amylase (alpha-1,4-glucan 4-glucanohydrolase, EC 3.2.1.1) of Bacillus subtilis strain W23 is less negatively-charged than the alpha-amylase of B. amyloliquefaciens strain F, as determined by electrophoretic mobility in polyacrylamide gel at pH 8.6. The alpha-amylase of strain W23 is immunologically unrelated to the alpha-amylase of strain F, as judged by lack of cross-reaction in Ouch...

Complete (Ba-L) and truncated (Ba-S) forms of alpha-amylases from Bacillus subtilis X-23 were purified, and the amino- and carboxyl-terminal amino acid sequences of Ba-L and Ba-S were determined. The amino acid sequence deduced from the nucleotide sequence of the alpha-amylase gene indicated that Ba-S was produced from Ba-L by truncation of the 186 amino acid residues at the carboxyl-terminal r...

Transcriptome analysis was used to investigate the global stress response of the gram-positive bacterium Bacillus subtilis caused by overproduction of the well-secreted AmyQ alpha-amylase from Bacillus amyloliquefaciens. Analyses of the control and overproducing strains were carried out at the end of exponential growth and in stationary phase, when protein secretion from B. subtilis is optimal....

Fermented succulent shoots of bamboo (Bambusa balcooa and Dendrocalamus strictus) are an enriched source of phytosterol. Microorganisms from the ‘soibum exudate’ involved in microbial bioconversion of phytosterol during fermentation of succulent bamboo shoots were isolated and identified as Bacillus subtilis, B. licheniformis, B. coagulans and Micrococcus luteus. Crude phytosterol was purified ...

The purposes of this research were to evaluate phorbol esters (PEs) degradation rate and enzyme production yield using submerged fermentation (SMF) as screening method and further using solid-state fermentation (SSF) as pilot scale-up study. SMF was carried out with 20 g seed cake in 100 ml minimal salt medium for 7 days incubation, while SSF was done with 20 g seed cake at 50% moisture content...

Owing to the production of alpha, beta and gamma amylase subtypes; starch degrading microbes, especially bacteria have an invincible role in the food, fermentation, textile and paper industries. Of them, α-amylases from Bacillus spp. have contributed tremendous advancements in bio-industry, especially in starch, detergent and pharmaceutical arena. Though general reviews are seen in literature o...

BACK GROUND For enzyme production, the costs of solid state fermentation (SSF) techniques were lower and the production higher than submerged cultures. A large number of fungal species was known to grow well on moist substrates, whereas many bacteria were unable to grow under this condition. Therefore, the aim of this study was to isolate a highly efficient strain of Bacillus sp utilizing wheat...

Amylases and cellulases are important enzymes both for the global carbon cycle on earth and for biotechnical applications. They are capable of degrading polysaccharides, which are chemically simple polymers of repeating glucose units that form very complex and water-insoluble macroscopic structures. The enzymatic degradation of starch and cellulose is poorly understood at the molecular level. T...