root rot disease of broad-bean caused by fusarium solani is a serious disease of this crop. due to high cost and difficulty of the disease control, interaction between root nodulating bacteria and fusarium. solani were evaluated to use for disease management. broad-bean root nodulating bacteria were isolated and their interaction with f. solani was investigated in culture medium in a completely...

An aerobic endospore-forming bacterium, tentatively identified as a strain (JJ-lb) of Bacillus macerans, was isolated by enrichment on 4-hydroxybenzoate (4HBA), using as an inoculum soil taken from a 50 degrees C Iadho hot spring. Enzymatic analyses of cells grown on succinate and 4HBA indicated that strain JJ-1b degrades 4HBA by way of the novel protocatechuate (PCA) 2,3-dioxygenase pathway. P...

background detection of fastidious enteropathogenic bacteria in fecal samples of patients with gastroenteritis is a challenge in clinical microbiological laboratories. objectives the aim of this study was to compare the detection limits of the pcr and culture methods for the diagnosis of campylobacter spp., yersinia spp., clostridium perfringens, and clostridium difficile in human stool samples...

Chemomechanical procedures can be used to eliminate bacteria from root canals. However, detectable bacteria sometimes remain because of the complexity of the root canal system. Endodontic passive ultrasonic irrigation (PUI) with hydrogen peroxide (H2O2) may be a promising option for increasing bactericidal hydroxyl radical (HO•) generation. In this in vitro experiment, we examined the effects o...

Currently, few techniques are available for the evaluation of bacterial biofilm adhesion. These detection tools generally require time for culture and/or arduous handling steps. In this work, the BioFilm Ring Test (BRT), a new technology, was used to estimate the biofilm formation kinetics of 25 strains of Pseudomonas aeruginosa, isolated from the sputum of cystic fibrosis (CF) patients. The pr...

bacillary dysentery, or shigellosis, is a disease of humans in which the colonic epithelium is invaded by bacteria and subjected to inflammatory destruction. the aim of this study was to develop a polymerase chain reaction(pcr) test for detection of virulent shigella spp.. for this purpose, the primers were designed to amplify a 526-bp internal region of the shigella spp. icsa gene, which encod...

The effect of a series of redox indicators and systems has been tested with a suspension of luminous bacteria (B. fischeri) in M/4 phosphate buffer of P(H) = 7.6. The indicators behave as expected from their position in the redox series, the most positive being reduced rapidly even in presence of air and before luminescence of the bacteria disappears, those of intermediate position at the time ...

The aerosol survival in air was determined for Pasteurella tularensis live vaccine strain (LVS) as a function of relative humidity (RH). Three different preparations of bacteria were used: (i) liquid suspension of P. tularensis LVS in spent culture medium; (ii) powders of P. tularensis LVS freeze-dried in spent culture fluid; (iii) P. tularensis LVS freeze-dried in spent culture fluid and then ...

Sponges are an important component of benthic ecosystems in New Zealand, and as suspension feeders they can process large volumes of water meaning that their interaction with the water column is likely to be of great importance. Our research focuses on the functional role of sponges as suspension feeders in temperate rocky subtidal ecosystems on the Wellington South Coast, New Zealand. We inves...