Listeria monocytogenes is a Gram-positive, psychrotropic, facultative anaerobic pathogen and it is the etiological agent of listeriosis, a severe foodborne disease of major public health concern. Listeria monocytogenes has high ability to adapt adverse condition. Therefore, the control of Listeria monocytogenes in foods and feeds is very difficult. N this purpose, organic acids and bacteriocins...

A novel bacteriocin, lactococcin MMFII, produced by Lactococcus lactis MMFII isolated from a Tunisian dairy product had been identified. The bacteriocin was purified to homogeneity from fresh overnight M17 broth culture by sulfate ammonium precipitation, cation-exchange chromatography, sep-pack chromatography and two steps of reverse-phase chromatography. The purified bacteriocin was heat stabl...

Sakacin G is a 37-amino-acid-residue-long class IIa bacteriocin produced by Lactobacillus sake 2512, which is encoded by the duplicated structural genes skgA1 and skgA2. Sakacin G appears to be unique and seems to be an intermediate between pediocin-like bacteriocins, according to its double-disulfide bridges required for antimicrobial activity, and mesentericin-like bacteriocins in terms of se...

Lactococcus lactis 61-14 isolated from river water produced a bacteriocin active against a wide range of Gram-positive bacteria. N-terminal amino acid sequencing, mass spectral analysis of the purified bacteriocin, and genetic analysis using nisin-specific primers showed that the bacteriocin was a new natural nisin variant, termed nisin Q. Nisin Q and nisin A differ in four amino acids in the m...

Lactococcin, a bacteriocin produced by Lactococcus lactis subsp. lactis ADRIA 85LO30, was purified as a 2.3-2.4 kDa peptide. Six non-bacteriocin-producing (Bac-) and non-immune (Imm-) strains were isolated after curing experiments. These strains had in common the loss or modification of two plasmids: pOS4 (32 kb) and pOS5 (70 kb). By comparing pOS5 and several modified plasmids, a DNA region fr...

Lactococcus lactis subsp. diacitilactis S50 produces a bacteriocin, designated bacteriocin S50, which has a narrow antibacterial spectrum. It was active only against Lactococcus species, including a nisin producer exhibiting a bactericidal effect. The activity of bacteriocin S50 was sensitive to proteases. It retained antimicrobial activity after being heated to 100 degrees C for up to 60 min a...

One of the lactic acid bacteria that has potential to produce crude bacteriocin is Pediococcus acidilactici BK01, which result isolated from Bekasam. Crude Bacteriocin BK01 a high-temperature resistance 121°C, indicated by antibacterial activity E. coli O15: H7 and S.aureus ATCC25923 extensive pH stability against Escherechia sensitive proteolytic enzymes. still shows during 30 days storage at ...

Bacteriocin JF246 has been purified from mitomycin C-induced Serratia marcescens cells by salt extraction, ammonium sulfate fractionation, and chromatography on QAE-Sephadex and SP-Sephadex. The purified material is homogeneous on polyacrylamide gel electrophoresis in the presence of 2% sodium dodecyl sulfate or 6 m urea. In the absence of these agents, the bacteriocin associates into aggregate...

A strain of Streptococcus mutans has been isolated that produces a bacteriocin with novel properties. Its antibiotic spectrum includes 123 of 124 strains of S. mutans tested and a variety of other gram-positive microorganisms. Experiments with dialysis membranes indicate that the molecular weight of the bacteriocin is less than 1,000. Mutants of the producer strain were obtained that are defici...

Chryseobacterium mulctrae KACC 21234T is a novel species isolated from raw bovine milk. Psychrotrophic bacteria are considered contaminants and hypothesized to originate the environment. In this investigation, C. genome was determined be 4,868,651 bp long assembled into four contigs with G+C ratio of 33.8%. silico genomic analyses revealed presence genes encoding proteases (endopeptidase Clp, o...