the dna molecule has been known to be the cellular target for many cytotoxic anticancer agents for several decades. understanding how drug molecules interact with dna has become an active research area in the interface between chemistry, molecular biology and medicine. dna extraction has been suggested as a main step affecting molecular dna technology such as pcr and pcr-based methods. therefor...

biochemical polymorphism study is a method of determination of genetic variation. this variability could be a basis for selection and subsequent genetic improvement in farm animals. the polymorphism in the intron 1 of chicken growth hormone (cgh) gene was investigated in the iranian native fowls by using polymerase chain reaction (pcr)-restriction fragment length polymorphism (rflp) method. the...

High litter size or twinning is an economically important trait that enhances sheep productivity. The FecXG has been influentially associated with the ovulation rate in various breeds. However, there limited information about this locus Kivircik breed. Therefore, aim of study was to evaluate presence Galway (FecXG) mutation (n=91) raised Kirklareli province. Genomic DNA isolated from whole bloo...

The main objective of the present study was to develop an efficient and reliable probe to be routinely used for detection of chromosome 13 copy numbers by interphase FISH. To achieve this, a Yeast Artificial Chromosome (YAC) containing sequences specific for human 13q12 (744D11), was cultured and the whole yeast genomic DNA was extracted. The human insert within the isolated DNA was amplified b...

the variation within a population of 100 pieces of native chicken from 5 regions of khuzestan province (abadan, dezful, shushtar, ahvaz, izeh) were analyzed by using 6 microsatellite markers, in the present study. the dna was extracted by using salting out method from blood samples collected. the pcr reactions for all locuses were performed and it was found that all of them are highly polymorph...

Gastrointestinal parasites are one of the main sources of economic decline in sheep production around the world. Resistant sheep do not completely reject the disease; they only harbor fewer parasites than susceptible sheep and therefore have a lower fecal egg count. The present research was designed to evaluate microsatellite polymorphism in intron 5 of DRB2 gene and its correlation with H. con...

The aim of the present study was to evaluate genotypic distribution calpastatin (CAST) and insulin-like growth factor 1 (IGF1) gene polymorphisms in Tsigai sheep breed. Genomic DNA isolated from blood samples by phenol-chloroform method. A total 56 were genotyped PCR-RFLP Allele genotype frequencies calculated standard procedures. To population-genetic properties, Hardy–Weinberg Equilibrium (HW...

This study was designed to identify genetically resistant animals to gastrointestinal nematode (GIN) infections using microsatellite polymorphisms of Ovar-DRB1 gene in Iranian Ghezel sheep breed lambs. In the present study 120 male Ghezel lambs were at 4 to 6 months of age randomly selected from six different sheep flocks in East Azerbaijan province (n=20 per flock). These lambs were naturally ...