obtaining a continuous source of normal cells or dna from a single individual has always been a rate limiting step in biomedical research. availability of lymphoblastoid cell lines (lcls) as a surrogate for isolated or cryopreserved peripheral blood lymphocytes has substantially accelerated the process of biological investigations. lcls can be established by in vitro infection of resting b cell...

Background Juvenile Dermatomyositis (JDM) is a systemic autoimmune inflammatory muscle disorder and vasculopathy that affects children younger than 16 years old. JDM was not associated with development of malignancies, unlike adult dermatomyositis. Nasopharynx carcinomas (NPC) are rare tumors, corresponding to 2% of tumors of the head and neck and 0,25% of all tumors. They are closely related t...

The Epstein-Barr virus-soluble (S) antigen extracted from RAJI cells was characterized by sucrose gradient centrifugation, gel filtration, and ion-exchange chromatography. The sedimentation coefficient was estimated to be 8.5S corresponding to a molecular weight of 180,000. The S antigen binds to DEAE-A25 ion exchanger from which it can be eluted with 0.3 M NaCl in Tris buffer (pH 7.2). All fra...

A 65,000-dalton (65K) antigen found in Raji cells by fluoroimmunoelectrophoresis and radioimmunoelectrophoresis has been identified as an Epstein-Barr virus nuclear antigen (EBNA). This identification is based on the following evidence. The 65K antigen is detected in Raji cells but not in three Epstein-Barr virus (-) human B cell lines. It is not detected with EBNA (-) sera. The 65K antigen is ...

Although often localized at diagnosis, nasopharyngeal carcinoma (NPC) has an established potential for distant metastasis. Breast metastasis from NPC is an uncommon presentation. In the present case study, the fifth reported case of breast metastasis from NPC is presented and the use of Epstein-Barr virus testing is demonstrated for the confirmation of this diagnosis. A 49-year-old female was d...

The viral infections with greatest impact on the renal transplant recipient are those due to cytomegalovirus, Epstein-Barr virus, and the two hepatitis viruses, hepatitis B and C. All of these are modulated by the administered immunosuppressive therapy, and all have both direct and indirect effects on the transplant patient. The direct effects are the infectious disease clinical syndromes that ...

Epstein-Barr virus, the apparent cause of infectious mononucleosis, may also be an etiological agent in nasopharyngeal carcinoma and Burkitt's lymphoma. Lymphocytes from normal individuals with anti-Epstein-Barr virus antibody activity may be sensitized to Epstein-Barr virus and contain transfer factor with the potential to program and/or recruit other lymphocytes to react against the virus and...

HEN stained with quinacrine o r nitrogen mustard derivatives of quinacrine, certain regions of Drosophila salivary gland chromosomes fluoresce with extraordinary brilliance (VOSA 1970; ELLISON and BARR 1971). Although the biochemical specificity of this reaction is not well understood, the method of quinacrine staining can be used for cytological comparisons of chromosomes among species and wit...

Lymphoepithelial carcinoma is a relatively uncommon malignant tumor of the salivary gland demonstrating malignant epithelial cells with dense lymphoid stroma. The authors report three cases of lymphoepithelial carcinoma associated with Epstein-Barr virus of the right parotid gland with clinically presenting as painless, gradual enlargement of the preauricular mass. The histopathologic examinati...

A monoclonal antibody (mAb) designated 55H3 was produced by using chemically induced Epstein-Barr virus genome-positive B95-8 cells. mAb 55H3, which reacted with an 85- to 80-kDa polypeptide, neutralized Epstein-Barr virus-encoded DNA polymerase activity in crude extracts of chemically induced M-ABA, HR-1, and B95-8 cells, as well as the partially purified Epstein-Barr virus DNA polymerase in a...