Protein interactions inside the human body are expected to differ from the situation in vitro. This is crucial when investigating protein functions or developing new drugs. In this study, we present a sample-efficient, free-solution method, termed microscale thermophoresis, that is capable of analysing interactions of proteins or small molecules in biological liquids such as blood serum or cell...

The macrolide antibiotic bafilomycin and the related synthetic compound SB 242784 are potent inhibitors of the vacuolar H+ -ATPases (V-ATPase). It is currently believed that the site of action of these inhibitors is located on the membrane bound c-subunits of V-ATPases. To address the identification of the critical inhibitors binding domain, their specific binding to a synthetic peptide corresp...

A [I]-BeKm-1 saturation binding assay was performed on hERG K channel membranes using FlashBlue beads. An average Bmax value of 0.6 pmol/mg (considering a counting efficiency of 40% for FlashBlue beads) was obtained, with a Kd value of 0.17 nM. Panel A shows a saturation binding curve. The corresponding Scatchard analysis is illustrated in panel B. The data presented is derived from single site...

One of the first critical steps in pathogenesis amoebic gill disease (AGD) farmed salmon is adhesion causative amoeba to host. The current study aimed investigate potential involvement glycan-binding proteins expressed on extracellular surface Neoparamoeba perurans tissue recognition and binding. properties membrane N. carbohydrate binding profile Atlantic gill-derived epithelial cells were ide...

Assessment and control of Fc functionality during biotherapeutic antibody development and manufacturing is essential as receptor binding is linked to the safety and efficacy of the final drug. Typically, cell-based assays (such as ADCC, ADCP, and CDC) and/or surrogate binding assays are used for routine monitoring or characterization of the Fc functionality. The use of Biacore ligand-binding as...

Objective(s): Somatostatin receptor-positive neuroendocrine tumors have been targeted using various peptide analogs radiolabeled with therapeutic radionuclides for years. The better biomedical properties of radioantagonists as higher tumor uptake make these radioligands more attractive than agonists for somatostatin receptor-targeted radionuclide therapy. In this study...

Androgen receptor (AR) mutations in androgen insensitivity syndrome (AIS) are associated with a variety of clinical phenotypes. The aim of the present study was to compare the molecular properties and potential pathogenic nature of 8 novel and 3 recurrent AR variants with a broad variety of functional assays. Eleven AR variants (p.Cys177Gly, p.Arg609Met, p.Asp691del, p.Leu701Phe, p.Leu723Phe, p...

Background: The affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as ELISA. Affinity of most IgG specific MAbs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...

Recent studies using solid-phase-binding assays and electron microscopy suggested the presence of a heparin-binding domain between the inner globule of a lateral short arm and the cross region of laminin. Using the information from the amino acid sequence of the B1 chain of laminin, several peptides were synthesized from areas with a low hydropathy index and a high density of lysines and/or arg...

SARS-CoV-2 contains a positive single-stranded RNA genome of approximately 30 000 nucleotides. Within this genome, 15 elements were identified as conserved between SARS-CoV and SARS-CoV-2. By nuclear magnetic resonance (NMR) spectroscopy, we previously determined that these fold independently, in line with data from vivo ex-vivo structural probing experiments. These contain non-base-paired regi...