A novel reversed-phase HPLC method has been developed and validated for the simultaneous determination of 2-phenoxyethanol, methylparaben, ethylparaben and propylparaben preservatives. The method uses a Lichrosorb C8 (150×4.6 mm, 5 µm) column and isocratic elution. The mobile phase consisted of a mixture of acetonitrile, tetrahydrofuran and water (21:13:66, v/v/v), pumped at a flow rate of 1 ml...

A stereoselective, sensitive and rapid liquid chromatography-tandem mass spectrometry (LCMSMS) method for the determination of R-acenocoumarol and S-acenocoumarol in human plasma was developed and validated at IPRC bioanalytical labs. The procedure involved solid phase extraction of both enantiomers and their corresponding internal standard. The chromatographic separation was accomplished emplo...

The principles and practice of the application of time-resolved lanthanide chelate luminescence (or fluorescence) as a detection method for ultrasensitive bioanalytical assays such as immunoassays and nucleic acid hybridization assays are reviewed. The various lanthanide chelate-based detection systems which have been developed for use in heterogeneous and homogeneous assay formats are describe...