Background. Mitochondrial respiratory chain (RC) disorders are a growing group of with large variety clinical presentations ranging from well-defined syndromes to nonspecific manifestations, such as failure thrive, exercise intolerance and seizures.Objective. To describe the clinical, biochemical, histochemical spectrum 38 Egyptian patients clinically suspected havingmitochondrial RC disorders....

Introduction: Since Curcuma longa extract and curcumin have been shown to be potent antioxidant agents, they were used in cultured adult mouse spinal cord slices to investigate whether they can inhibit apoptosis in motor neurons. Methods: Slices from the thoracic region of adult mice spinal cord were divided into four groups: 1. Freshlyprepared slices (time 0) 2. Control 3. Slices treated by cu...

BACKGROUND Human serum albumin (HSA) is the most abundant plasma protein and plays key a role in metabolism. The variation in albumin concentration provides valuable information related to metabolic diseases and diagnostic application. METHODS We constructed two assay systems to quantify the albumin concentration. The immunoassay used a fluorescence (FL) dye to detect albumin in samples and e...

Small molecules have been identified as potential therapeutic agents for lysosomal storage diseases (LSDs), inherited metabolic disorders caused by defects in proteins that result in lysosome dysfunctional. Some small molecules function assisting the folding of mutant misfolded lysosomal enzymes that are otherwise degraded in ER-associated degradation. The ultimate result is the enhancement of ...

Empirical Kinetic Models have been used to describe and establish the Anaerobic Digestion kinetics of pig slurry’s (8% TS) Biochemical Chemical Potential. A wide selection were fitted experimental data collected in batch assays different Substrate Inoculum ratios, 0.65 (BMP1) 1 (BMP2). For most suitable model for each BMP assay, statistical tools R2 RMSE, along with Information Criterion AIC BI...

Point-of-care (POC) biochemical assay is a highly important to estimate hemoglobin in the blood. High reagent volumes and complex-expensive optical setup requirements pose serious challenges when it comes adopting conventional assays such as Sodium Lauryl Sulfate (SLS) method into POC device. Here, we report modified SLS on microfluidic platform, wherein quantification achieved using simple mic...