The effects of non-ionic surfactants as mobile phase additives on the fluorescence intensity of dansyl derivatives of biogenic amines in high-performance thin-layer chromatography (HPTLC) were investigated. The dansyl derivatives of putrescine, cadaverine, spermidine and spermine were separated by HPTLC with silica gel as stationary phase and an eluent consisting of chloroform–triethylamine (2 ...

This study was carried out to evaluate the quality of the three edible tissues (muscles, hepatopancreas and gonads) of the male and female Chinese mitten crab (Eriocheir sinensis) in natural harvest season, based on total volatile base nitrogen test (TVBN) and biogenic amines analysis at intervals time of 3 h extended for 24 h during 30 °C storage (a mean ambient temperature in harvest season)....

This manuscript offers a comprehensive review about the bacterial amino acid decarboxylase activity and biogenic amines content of an artisanal cheese produced in Italy, named as Pecorino cheese. It is highlighted the importance of controlling primary production of raw milk, as well as cheese-making process to avoid the release of free amino-acids and viability of microorganisms possessing amin...

The microbial decarboxylation of some amino acids leads to the undesirable presence of biogenic amines in foods. One of the most abundant and frequent biogenic amines found in fermented foods is tyramine, which is produced by the decarboxylation of tyrosine. In the present work, transcriptional analysis of tyramine biosynthesis in Enterococcus durans IPLA655, a strain isolated from cheese, was ...

Electrochemistry Discover the difference Summary The ALEXYS Neurotransmitter Analyzer is a modular system for UHPLC with electrochemical detection of neurotransmitters. This application note shows the applicability of the system to measure various biogenic amines in on-line microdialysis (OMD) which is a continuously flowing microdialysis sampling setup. Instead of collecting samples and puttin...

2,4-Toluene diamine (TDA), a class A carcinogen, is a major raw material for the production of toluene diisocyanate (TDI), which is one of the precursors for the production of polyurethane foams (PU). This review deals with 2,4-toluene diamine's (TDA) carcinogenicity, analytical techniques, biodegradation and use as a biosensor for biogenic and synthetic amines, emphasizing various carcinogenic...

The failure to adapt metabolism to the homeorhetic demands of lactation is considered as a main factor in reducing the productive life span of dairy cows. The so far defined markers of production performance and metabolic health in dairy cows do not predict the length of productive life span satisfyingly. This study aimed to identify novel pathways and biomarkers related to productive life in d...

Biogenic Amines (BAs) are low molecular weight organic bases with biological activity that are formed in foods by either microbial decarboxylation of the corresponding amino acids or transamination of aldehydes and ketones by amino acid transaminases [1]. The most important BAs including histamine, tyramine, tryptamine, putrescine, and cadaverine are formed from free amino acids namely histidin...

Vesicular monoamine transporters (VMATs) are a prerequisite for the uptake of biogenic amines into intracellular storage organelles, whereas soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs; such as SNAP-25 and syntaxin1) are essential for exocytosis of biogenic amines by neurons and endocrine cells. In this study, we examined whether these proteins exist in high-g...