Mycotoxin contamination of food and feedstuffs is among the top priorities for human and animal safety. The currently used techniques for mycotoxin determination, either chromatography or ELISA, are unsuitable for routine in-field assessment. There is an urgent need for other accurate, simple and cost-effective techniques that can be used as a screening tool for a rapid estimation of mycotoxin ...

Spectrally-resolved BioLuminescence optical Tomography (sBLT) is used to recover images of Luciferase activity within a 3D model using multi-wavelength emission data from internal bioluminescence sources. The Adjoint theorem for 3D image reconstruction is presented and validated using experimental data with a complete computation time of 5 minutes. ©2006 Optical Society of America OCIS codes: (...

Chemiand bioluminescence measurements have become extremely popular in recent years. They are often used to determine the amount of a specific unknown present in a sample, and in the last decade, have become extremely important in the study of gene expression and gene regulation. Chemiluminescence is the light emitted by a chemical reaction and bioluminescence is a type of chemiluminescence in ...

Spectrally-resolved BioLuminescence optical Tomography (sBLT) is used to recover images of Luciferase activity within a 3D mouse model using multi-wavelength emission data from multiple internal bioluminescence sources. The images show that the location of sources can be reconstructed accurately using a fast, direct and linear reconstruction algorithm. ©2006 Optical Society of America OCIS code...

The luxA,B genes from the Gram-negative marine bacterium Vibrio harveyi MAV were used in Staphylococcus carnosus TM300 as a reporter system for regulated expression of xylose utilization. The luciferase genes were fused to the xyl operon from Staphylococcus xylosus C2a. Expression of bioluminescence was induced through addition of xylose and repressed in the presence of glucose. A method to qua...

We report bioluminescence analysis of matrix metalloproteinase (MMP) activity in biological substances using a surface-bound luciferase probe. Intein-fused luciferase protein enables site-specific biotinylation of luciferase in the presence of N-terminus cysteine-biotin via intein-mediated splicing process, resulting in a strong association with high bioluminescence signal onto a NeutrAvidin-co...