In vivo imaging of cells tagged with light-emitting probes, such as firefly luciferase or fluorescent proteins, is a powerful technology that enables a wide range of biological studies in small research animals. Reporters with emission in the red to infrared (>600 nm) are preferred due to the low absorption in tissue at these wavelengths. Modeling of photon diffusion through tissue indicates th...

The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. Sepsis remains the major cause of death in patients with major burn injuries. In the present investigation we evaluated the interaction between burn injuries of varying severity and preexisting distant infection. We used Gram-negative bacteria (Pseudomonas aeruginosa and Proteu...

The glacier lanternfish Benthosema glaciale is a key myctophid with wide distribution in the northern Atlantic. It species that has strong vertical migration capacity and have potential to move between surface waters mesopelagic depths diel cycle (DVM), mainly depending on ambient light conditions. We investigated feeding ecology of B. across Norwegian, Iceland, Irminger Labrador Sea basins. An...

Abstract Keroplatus is a genus of fungus gnats family Keroplatidae (Diptera, Bibionomorpha). Larvae some species emit constant blue light from the body. The bioluminescence one least studied all terrestrial insects and very few facts are known to date its biology biochemistry. Here we report high level riboflavin in testaceus larvae, fluorescent compound that might be relative bioluminescent sy...

Graphical Abstract The cover picture shows the colonies of bioluminescent bacteria that Bonnie Bassler uses to decipher molecular mechanism governing microbial chemical communication. Also shown are several key molecules involved in As highlighted throughout this special issue, work on communication has strong impact both basic and applied microbiology. was designed by Julia van Kessel photogra...

Video images of biohiminescence were recorded in situ during a 1985 study of the midwater environment of the Monterey Canyon, using a single-person, untethered submersible. Gelatinous organisms were responsible for the most brilliant bioluminescent displays, often exhibiting elaborate kinetics in response to mechanical stimulation. Images of bioluminescent displays recorded from identified orga...

We describe the system design of the first bioluminescence tomography (BLT) system for parallel acquisition of multiple bioluminescent views around a mouse in a number of spectral channels simultaneously. The primary component of this BLT system is a novel mirror module and a unique mouse holder. The mirror module consists of a mounting plate and four mirrors with stages. These mirror stages ar...

This paper applies computer vision techniques to underwater video images of bioluminescent biota for quantifying, tracking, and identification. Active contour models are adapted for computerized image segmentation, labeling, tracking, and mapping of the bioluminescent plankton recorded by lowlight-level video techniques. The system automatically identifies luminous events and extracts features ...

After activation, populations of antigen-specific T cells flow between sites of antigen expression, local lymphoid structures and other lymphoid and non-lymphoid organs. In this study, we documented the in vivo dynamics of a CD8(+) T cell response to antigen delivered using herpes simplex virus amplicon vectors and revealed several unexpected features. First, the T cells localized to the site o...

We introduce a general method to transform antibodies into ratiometric, bioluminescent sensor proteins for the no-wash quantification of analytes. Our approach is based on the genetic fusion of antibody fragments to NanoLuc luciferase and SNAP-tag, the latter being labeled with a synthetic fluorescent competitor of the antigen. Binding of the antigen, here synthetic drugs, by the sensor displac...