The archaeon Haloferax mediterranei was selected for production of PHA co- and terpolyesters using inexpensive crude glycerol phase (CGP) from biodiesel production as carbon source. CGP was assessed by comparison with the application of pure glycerol. Applying pure glycerol, a copolyester with a molar fraction of 3-hydroxybutyrate (3HB) of 0.90 mol/mol and 3-hydroxyvalerate (3HV) of 0.10 mol/mo...

nowadays, hydrogels are widely used as wound dressings in biomedical applications. similar to other types of the moist (wet) wound dressings, it is necessary to have sufficient information about their dehydration kinetics during the pre-usage period (e.g. storage duration) and also the wound healing process. in this work, hydrogel wound dressings based on polyvinyl alcohol were prepared by cycl...

The production of polyhydroxyalkanoates (PHA; bioplastics) from waste or surplus feedstocks using mixed microbial consortia (MMC) and aerobic dynamic feeding (ADF) is a growing field within mixed culture biotechnology. This study aimed to optimize a 2DE workflow to investigate the proteome dynamics of an MMC synthesizing PHA from fermented dairy manure. To mitigate the challenges posed to effec...

1. ABSTRACT Polyhydroxyalkanoates are excellent polymeric materials for production of environmentally degradable packaging devices and have potential applicability in medical uses. Aim of this project was the isolation of PHA from fermentation broth. Two general approaches for isolation of biopolymers from fermentation broth have been determined and compared. The first possibility is a multista...

Research on biodegradable biopolymers has been increasing rapidly during the past few decades due to the environmental concerns related to plastic pollution. Polyhydroxyalkanoates (PHAs), the structurally versatile, biodegradable polyoxoesters produced by diverse group of microorganisms as intracellular storage granules have material properties similar to thermoplastics and identified as a prom...

The combined fermentation of sludge from the secondary sedimentation tank of municipal wastewater treatment plants and food waste enables not only waste reduction, but also the acquisition of volatile fatty acids (VFAs) for the biosynthesis of polyhydroxyalkanoates (PHAs). To better understand variables influencing the production of VFAs and PHAs, this study considered anaerobic fermentation of...

The use of plastic materials is forecasted to double by 2030 in Africa. increase demand presents an opportunity develop biopolymers such as polyhydroxyalkanoates (PHA) instead petroleum-based plastics. However, the high cost PHA production closely linked feedstock price, which will hinder their deployment. On other hand, Sub-Saharan Africa (SSA) economy heavily reliant on agriculture, with cass...

Fibreboard production is one of the most important industrial activities in Galicia (Spain). Great amounts of wastewater are generated, with properties depending on the type of wood, treatment process, final product and water reusing, among others. These effluents are characterized by a high chemical oxygen demand (COD), low pH and nutrients limitation. Aerobic and anaerobic processes have been...

Abstract Over the decades, global plastic production has been exponentially increasing with a significant increase of waste as well. Consequently, our environment suffered lot because synthetic is less biodegradable or even not completely biodegradable. On other hand, conventional recycling rate and management in top ten contributors are still low to reduce contamination pollution from waste. P...

Because of their lower environmental impact, biodegradable polymers such as polyhydroxyalkanoates (PHAs) produced within cultured bacteria represent promising alternatives to petroleum-based plastics. PHA production in flasks yielded optimal results with a carbon-to-nitrogen ratio 22. The 5-L scale experimental revealed that when glucose was used the carbon source, Cupriavidus necator could pro...